beta Tubulin Antibody (D-10): sc-5274

β Tubulin Antibody (D-10) is a mouse monoclonal IgG2b kappa light chain antibody engineered to recognize β Tubulin, also known as tubulin beta chain, across mouse, rat, and human species. β Tubulin monoclonal antibody (D-10) is raised against a recombinant protein corresponding to amino acids 210-444 of human β Tubulin, ensuring precise binding and robust performance in applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). β Tubulin is a fundamental component of the cytoskeleton, playing a crucial role in maintaining cell structure, facilitating intracellular transport, and enabling cell division through the formation and stabilization of microtubules. β Tubulin exists in multiple isoforms, including β1, β2, β3, β4, β5, β6, and β8, each with unique functions and cellular localizations, such as the neuron-specific β3 isoform. This diversity allows β Tubulin to participate in various cellular processes, making β Tubulin an essential target for research in areas like neurobiology, cancer, and cell biology. Understanding β Tubulin′s function is vital for elucidating mechanisms of cell motility, division, and intracellular organization, which are fundamental to both normal physiology and disease states. β Tubulin (D-10) antibody is available in both non-conjugated form and multiple conjugated forms, including horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and various Alexa Fluor® conjugates, providing versatility for different experimental setups.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

beta Tubulin Antibody (D-10) References:

1. Presence of the betaII isotype of tubulin in the nuclei of cultured mesangial cells from rat kidney.  |  Walss, C., et al. 1999. Cell Motil Cytoskeleton. 42: 274-84. PMID: 10223634
2. Identification of betaIII- and betaIV-tubulin isotypes in cold-adapted microtubules from Atlantic cod (Gadus morhua): antibody mapping and cDNA sequencing.  |  Modig, C., et al. 1999. Cell Motil Cytoskeleton. 42: 315-30. PMID: 10223637
3. Delta-tubulin and epsilon-tubulin: two new human centrosomal tubulins reveal new aspects of centrosome structure and function.  |  Chang, P. and Stearns, T. 2000. Nat Cell Biol. 2: 30-5. PMID: 10620804
4. Tubulin immunoreactive neuronal intranuclear inclusions in the human brain.  |  Woulfe, J. and Munoz, D. 2000. Neuropathol Appl Neurobiol. 26: 161-71. PMID: 10840279
5. Alpha-, beta-, and gamma-tubulins: sequence comparisons and structural constraints.  |  Burns, RG. 1991. Cell Motil Cytoskeleton. 20: 181-9. PMID: 1773446
6. Gamma-tubulin is present in Drosophila melanogaster and Homo sapiens and is associated with the centrosome.  |  Zheng, Y., et al. 1991. Cell. 65: 817-23. PMID: 1904010
7. Invited review: the role of nucleotide triphosphate in actin and tubulin assembly and function.  |  Weisenberg, RC. 1981. Cell Motil. 1: 485-97. PMID: 6756643
8. Multiple forms of tubulin: different gene products and covalent modifications.  |  Ludueña, RF. 1998. Int Rev Cytol. 178: 207-75. PMID: 9348671
9. Expression of amino- and carboxyl-terminal gamma- and alpha-tubulin mutants in cultured epithelial cells.  |  Leask, A. and Stearns, T. 1998. J Biol Chem. 273: 2661-8. PMID: 9446570

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-beta Tubulin Antibody (D-10) has 585 citations in a variety of scientific publications.

Primary antibodies like Anti-beta Tubulin Antibody (D-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

beta Tubulin Antibody (D-10) Specifications

Alternate Product Name: β Tubulin

Product Clone Name: D-10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 210-444

Epitope Target: β Tubulin

Epitope Species: human

Product Reactivity/Detection: β Tubulin

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 585 citations in various scientific publications

Available Conjugates/Forms for beta Tubulin Antibody (D-10): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, B: Biotin, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin