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home » Phospho-ThreonineAntibody to Phosphorylated Amino Acids / Antibodies to Phosphorylated Amino Acids » p-Thr (H-2): sc-5267

p-Thr (H-2) Antibody: sc-5267

  |  Datasheet
  • mouse monoclonal IgG1; 200 µg/ml
  • specific detection of phosphothreonine-containing proteins; non cross-reactive with phosphotyrosine or phosphoserine by WB, IP, IF, IHC(P), FCM and ELISA
  • agarose conjugate for IP studies, sc-5267 AC, 500 µg/0.25 ml agarose
  • HRP conjugate; sc-5267 HRP, 200 µg/ml
  • fluorescein conjugate for immunofluorescence; sc-5267 FITC, 200 µg/ml
  • biotin conjugate, sc-5267 B, 200 µg/ml
  • phycoerythrin (sc-5267 PE) conjugate for intracellular FCM, 100 tests
  • Alexa Fluor® 405 (sc-5267 AF405), Alexa Fluor® 488 (sc-5267 AF488) and Alexa Fluor® 647 (sc-5267 AF647) conjugates for FCM or immunofluorescence; 100 µg/2 ml
  • blocking peptide, sc-5267 P
 
Additional Antibodies to Phosphorylated Amino Acids ...
 
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Recommended Support Products:
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WB   IP   IF   IHC(P)   FCM   siRNA  
Set Currency

 Ordering Information
Product NameCatalog #UnitPriceQtyAddFavorites
p-Thr (H-2) sc-5267 200 µg/ml $279
p-Thr (H-2) AC sc-5267 AC 500µg/ml, 25%ag $366
p-Thr (H-2) Alexa Fluor® 405 sc-5267 AF405 100 µg/2 ml $314
p-Thr (H-2) Alexa Fluor® 488 sc-5267 AF488 100 µg/2 ml $314
p-Thr (H-2) Alexa Fluor® 647 sc-5267 AF647 100 µg/2 ml $314
p-Thr (H-2) B sc-5267 B 200 µg/ml $282
p-Thr (H-2) FITC sc-5267 FITC 200 µg/ml $282
p-Thr (H-2) HRP sc-5267 HRP 200 µg/ml $279
p-Thr (H-2) P sc-5267 P
(peptide)
 100 µg/0.5 ml $61
p-Thr (H-2) PE sc-5267 PE 100 tests in 2ml $303
 WB Positive Control Cell Lysate (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
NIH/3T3 Whole Cell Lysate sc-2210 500 µg/200 µl $104

Thr Background Information
Protein kinases catalyze the phosphorylation of serine, threonine or tyrosine residues in target substrates, providing a mechanism of control for myriad cellular signaling pathways. Threonine phosphorylation plays a role in the activation of ERK and JNK MAP kinases, which are dually phosphorylated on tyrosine and threonine residues by MEK family kinases (1,2). Several families of kinases phosphorylate both serine and threonine residues in target substrates, including the Raf, Rsk, ROCK, PAK, Ak, and PKC families of protein kinases (3–8). Antibodies to phosphothreonine may be used for the characterization of proteins with phosphorylated threonine residues, and for the elucidation of cellular pathways involving threonine phosphorylation.

p-Thr (H-2) Product Citations
See how others have used p-Thr (H-2): sc-5267 antibody and or p-Thr (H-2) antibody conjugates.


22 total citations
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Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
 
p-Thr (H-2)
Click on image to enlarge
sc-5267
p-Thr (H-2): sc-5267. Western blot analysis of threonine phosphorylated proteins in whole cell lysates from control NIH/3T3 cells (A) and NIH/3T3 cells treated with IL-6 (B).
sc-5267
p-Thr (H-2) PE: sc-5267 PE. Intracellular FCM analysis of fixed and permeabilized NIH/3T3 cells. Black line histogram represents the isotype control, normal mouse IgG1: sc-2866.
sc-5267
p-Thr (H-2): sc-5267. Immunoperoxidase staining of formalin fixed, paraffin-embedded human breast tumor (A) and normal human breast tissue B) each showing cytoplasmic localization.
sc-5267
p-Thr (H-2): sc-5267. Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing cytoplasmic localization.
sc-5267
p-Thr (H-2) AF488: sc-5267 AF488. Intracellular FCM analysis of fixed and permeabilized NIH/3T3 cells. Black line histogram represents the isotype control, normal mouse IgG1: sc-3890.
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