epitope mapping near the C-terminus of Rbx1 of human origin
recommended for detection of Rbx1 of mouse, rat and human origin by WB, IF and ELISA; also reactive with additional species, including equine, canine, bovine and avian
Rbx1 Background Information Rbx1 (also designated ROC1 and Hrt1) and the closely related protein Rbx2 (also designated ROC2) are RING finger containing homologs of the yeast protein APC11, a member of the anaphase-promoting complex (APC) (1-4). Rbx1 was shown to be a component of the von Hippel-Lindau (VHL) transcription elongation complex, which includes VHL, Elongin B, Elongin C and Cullin-2 (1). Rbx1 interacts with Cullin-1 in the SCF
(Skp1-Cdc53-F-box protein) ubiquitin ligase complex (2-4). Rbx1 functions as a common subunit of SCF complexes required for ubiquination of various proteins including yeast G1 cyclins, IkBa and b-catenin (2-4). Rbx1 was shown to enhance the ubiquitin ligase activity of the VHL/Cullin-2 complex, and of the SCF/Cullin-1 complex (5).
