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- rabbit polyclonal IgG, 200 µg/ml; also available as goat IgG, 200 µg/ml, sc-52-G
- epitope mapping at the N-terminus of c-Fos of human origin
- recommended for detection of c-Fos of mouse, rat and human origin by WB, IP, IF, IHC(P), FCM and ELISA; also reactive with additional species, including equine and feline
- blocking peptide, sc-52 P
- HRP conjugate, sc-52 HRP, 200 µg/ml
- fluorescein (sc-52 FITC) and rhodamine (sc-52 TRITC) conjugates for immunofluorescence, 200 µg/ml
- phycoerythrin conjugate for intracellular FCM, sc-52 PE, 100 tests
- TransCruz reagent for Gel Supershift and ChIP applications, sc-52 X, 200 µg/0.1 ml
- Alexa Fluor® 405 (sc-52 AF405), Alexa Fluor® 488 (sc-52 AF488) and Alexa Fluor® 647 (sc-52 AF647) conjugates for FCM or immunofluorescence; 100 µg/2 ml
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c-Fos Background Information The c-Fos oncogene was initially detected in two independent murine osteosarcoma virus isolates and an avian nephroblastoma virus. The cellular homolog, c-Fos, encodes a nuclear phospho-protein that is rapidly and transiently induced by a variety of agents and functions as a transcriptional regulator for several genes. In contrast to c-Jun proteins, which form homo- and heterodimers which bind to specific DNA response elements, c-Fos proteins are only active as heterodimers with members of the Jun gene family. Functional homologs of c-Fos include the Fra-1, Fra-2 and Fos B genes. In addition, selected ATF/CREB family members can form leucine zipper dimers with Fos and Jun. Different dimers exhibit differential specificity and affinity for AP-1 and CRE sites.
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c-Fos (4)
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c-Fos (4): sc-52. Nuclear immunofluorescence staining of methanol-fixed, phorbol ester-induced HeLa cells.
c-Fos (4) PE: sc-52 PE. Intracellular FCM analysis of fixed and permeabilized control (green line histogram) and EGF transfected (solid orange histogram) A-431 cells. Dotted pink histogram represents the isotype control, normal rabbit IgG: sc-3871.
c-Fos siRNA (h): sc-29221. Western blot analysis of c-Fos expression in non-transfected control (A) and c-Fos siRNA transfected (B) A-431 cells. Blot probed with c-Fos (4): sc-52. Actin (I-19): sc-1616 used as specificity and loading control.
c-Fos (4) PE: sc-52 PE. Intracellular FCM analysis of fixed and permeabilized MIA PaCa-2 cells. Black line histogram represents the isotype control, normal rabbit IgG: sc-3871.
c-Fos (4) Alexa Fluor 488: sc-52 AF488. Intracellular FCM analysis of fixed and permeabilized MIA PaCa-2 cells. Black line histogram represents the isotype control, normal rabbit IgG: sc-45068.
c-Fos (4) Alexa Fluor 647: sc-52 AF647. Intracellular FCM analysis of fixed and permeabilized MIA PaCa-2 cells. Black line histogram represents the isotype control, normal rabbit IgG: sc-24647.
c-Fos (4)-G: sc-52-G. Western blot analysis of c-Fos expression in untreated (A) and phorbol ester-treated (B) nuclear cell extracts.
c-Fos (4): sc-52. Western blot analysis of c-Fos expression in non-transfected 293T: sc-117752 (A), mouse c-Fos transfected 293T: sc-118888 (B) and Jurkat (C) whole cell lysates.
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