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c-Fos (4) Antibody: sc-52

 |  Datasheet
  • rabbit polyclonal IgG, 200 µg/ml; also available as goat IgG, 200 µg/ml, sc-52-G
  • epitope mapping at the N-terminus of c-Fos of human origin
  • recommended for detection of c-Fos of mouse, rat and human origin by WB, IP, IF, IHC(P), FCM and ELISA; also reactive with additional species, including equine and feline
  • blocking peptide, sc-52 P
  • HRP conjugate, sc-52 HRP, 200 µg/ml
  • fluorescein (sc-52 FITC) and rhodamine (sc-52 TRITC) conjugates for immunofluorescence, 200 µg/ml
  • phycoerythrin conjugate for intracellular FCM, sc-52 PE, 100 tests
  • TransCruz reagent for Gel Supershift and ChIP applications, sc-52 X, 200 µg/0.1 ml
  • Alexa Fluor® 405 (sc-52 AF405), Alexa Fluor® 488 (sc-52 AF488) and Alexa Fluor® 647 (sc-52 AF647) conjugates for FCM or immunofluorescence; 100 µg/2 ml
 
Additional c-Fos Antibodies ...
 
Ordering InformationProduct Citations
Recommended Support Products:
(click button of application of choice)
WB   IP   IF   IHC(P)   FCM   Gel Shift   ChIP   siRNA  
 
Species Gene Name Gene ID Chromosome Location Isoform (mRNA) Accession # Protein Accession # OMIM™ Number
Human FOS 2353 14q24.3 NM_005252 P01100
164810
Human FOS 2353 14q24.3 P01100
164810
Mouse Fos 14281 12 D2 P01101
N/A
Mouse Fos 14281 12 D2 P01101
N/A
 
Set Currency

 Ordering Information
Product NameCatalog #UnitPriceQtyAddFavorites
c-Fos (4) sc-52 200 µg/ml $279
c-Fos (4) Alexa Fluor® 405 sc-52 AF405 100 µg/2 ml $314
c-Fos (4) Alexa Fluor® 488 sc-52 AF488 100 µg/2 ml $314
c-Fos (4) Alexa Fluor® 647 sc-52 AF647 100 µg/2 ml $314
c-Fos (4) FITC sc-52 FITC 200 µg/ml $282
c-Fos (4) HRP sc-52 HRP 200 µg/ml $279
c-Fos (4) P sc-52 P
(peptide)
100 µg/0.5 ml $61
c-Fos (4) TRITC sc-52 TRITC 200 µg/ml $282
c-Fos (4) X sc-52 X 200 µg/0.1 ml $279
c-Fos (4)-G sc-52-G 200 µg/ml $279
 siRNA Gene Silencers (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
c-Fos siRNA (h) sc-29221 10 µM $258
c-Fos siRNA (m) sc-29222 10 µM $258
c-Fos siRNA (h2) sc-44200 10 µM $258
c-Fos (h)-PR sc-29221-PR 10 µM $23
c-Fos (m)-PR sc-29222-PR 10 µM $23
c-Fos (h2)-PR sc-44200-PR 10 µM $23
 shRNA Plasmids (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
c-Fos shRNA Plasmid (h) sc-29221-SH 20 µg $520
c-Fos shRNA Plasmid (m) sc-29222-SH 20 µg $520
c-Fos shRNA Plasmid (h2) sc-44200-SH 20 µg $520
 shRNA Lentiviral Particles (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
c-Fos shRNA (h) Lentiviral Particles sc-29221-V 200 µl $625
c-Fos shRNA (m) Lentiviral Particles sc-29222-V 200 µl $625
c-Fos shRNA (h2) Lentiviral Particles sc-44200-V 200 µl $625
 WB Positive Control Cell Lysates (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
A-431 nuclear extract sc-2122 1000 µg $143
Jurkat Whole Cell Lysate sc-2204 500 µg/200 µl $104
A-431 + PMA nuclear extract sc-2123 1000 µg $143
A-431 + EGF nuclear extract sc-2124 1000 µg $143
Y79 nuclear extract sc-2126 1000 µg $143
c-Fos (m): 293T Lysate sc-118888 100µg/200µl $205
Fos B (h): 293T Lysate sc-112170 100µg/200µl $205

c-Fos Background Information
The c-Fos oncogene was initially detected in two independent murine osteosarcoma virus isolates and an avian nephroblastoma virus. The cellular homolog, c-Fos, encodes a nuclear phospho-protein that is rapidly and transiently induced by a variety of agents and functions as a transcriptional regulator for several genes. In contrast to c-Jun proteins, which form homo- and heterodimers which bind to specific DNA response elements, c-Fos proteins are only active as heterodimers with members of the Jun gene family. Functional homologs of c-Fos include the Fra-1, Fra-2 and Fos B genes. In addition, selected ATF/CREB family members can form leucine zipper dimers with Fos and Jun. Different dimers exhibit differential specificity and affinity for AP-1 and CRE sites.

c-Fos (4) Product Citations
See how others have used c-Fos (4): sc-52 antibody and or c-Fos (4) antibody conjugates.


313 total citations
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c-Fos (4)
Click on image to enlarge
c-Fos (4): sc-52. Nuclear immunofluorescence staining of methanol-fixed, phorbol ester-induced HeLa cells.
c-Fos (4) PE: sc-52 PE. Intracellular FCM analysis of fixed and permeabilized control (green line histogram) and EGF transfected (solid orange histogram) A-431 cells. Dotted pink histogram represents the isotype control, normal rabbit IgG: sc-3871.
c-Fos siRNA (h): sc-29221. Western blot analysis of c-Fos expression in non-transfected control (A) and c-Fos siRNA transfected (B) A-431 cells. Blot probed with c-Fos (4): sc-52. Actin (I-19): sc-1616 used as specificity and loading control.
c-Fos (4) PE: sc-52 PE. Intracellular FCM analysis of fixed and permeabilized MIA PaCa-2 cells. Black line histogram represents the isotype control, normal rabbit IgG: sc-3871.
c-Fos (4) Alexa Fluor 488: sc-52 AF488. Intracellular FCM analysis of fixed and permeabilized MIA PaCa-2 cells. Black line histogram represents the isotype control, normal rabbit IgG: sc-45068.
c-Fos (4) Alexa Fluor 647: sc-52 AF647. Intracellular FCM analysis of fixed and permeabilized MIA PaCa-2 cells. Black line histogram represents the isotype control, normal rabbit IgG: sc-24647.
c-Fos (4)-G:sc-52-G. Western blot analysis of c-Fos expression in untreated (A) and phorbol ester-treated (B) nuclear cell extracts.
c-Fos (4): sc-52. Western blot analysis of c-Fos expression in non-transfected 293T: sc-117752 (A), mouse c-Fos transfected 293T: sc-118888 (B) and Jurkat (C) whole cell lysates.
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