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- mouse monoclonal IgG1, 200 µg/ml
- raised against p21 protein of human origin
- recommended for detection of p21 of mouse and human origin by WB, IP, IF and IHC(P)
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p21 Background Information
It is now well established that cyclins play a positive role in promoting cell cycle transitions via their ability to associate with and activate their cognate cyclin-dependent kinases (Cdks). Cdk2 associates with cyclins A, D and E, and has been implicated in the control of the G1 to S phase transition in mammals. A novel Cdk-interacting protein, designated p21, Cip1 or WAF1, has been identified in cyclin A, cyclin D1, cyclin E and Cdk2 immunoprecipitates. p21 is a potent, tight-binding inhibitor of Cdks and can inhibit the phosphorylation of Rb by cyclin A-Cdk 2, cyclin E-Cdk2, cyclin D1-Cdk4 and cyclin D2-Cdk4 complexes. Expression of p21 is inducible by wildtype, but not mutant, p53. The mouse homolog of p21 is designated CAP20. |
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p21 (WA-1)
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p21 (WA-1): sc-51689. Western blot analysis of p21 expression in untreated C32 (A) and PMA treated C32 (B) nuclear extracts.
p21 (WA-1): sc-51689. Western blot analysis of p21 expression in non-transfected 293T: sc-117752 (A), mouse p21 transfected 293T: sc-122305 (B) and C32 (C) whole cell lysates. p21 (WA-1): sc-51689. Western blot analysis of p21 expression in HeLa nuclear extract.
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