ERK 1/2 Antibody (C-9): sc-514302

ERK 1/2 Antibody (C-9) is a mouse monoclonal IgG2a kappa light chain antibody that detects ERK 1/2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). ERK 1/2 (C-9) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. ERK 1/2 proteins, also known as extracellular-signal-regulated kinases, play a crucial role in mitogen-activated protein kinase (MAPK) signaling pathways, which are essential for transmitting signals from growth factors, steroid hormones, and neurotransmitters to the nucleus of the cell. ERK 1/2 kinases are primarily located in the cytoplasm but translocate to the nucleus upon activation, where ERK 1/2 regulate various cellular processes such as proliferation, differentiation, and transcription. Phosphorylation of ERK 1 and ERK 2 by upstream kinases like MAP kinase kinase (MEK) is vital for activation, and this process is tightly regulated through specific consensus motifs. Human ERK 1 gene is located on chromosome 16p11.2 and encodes a 379 amino acid protein that exhibits 83% sequence identity with ERK 2, highlighting the evolutionary conservation and functional importance of these kinases in cellular signaling pathways. These attributes make anti-ERK 1/2 antibody (C-9) an invaluable tool for studying MAPK signaling pathways and their roles in regulating essential cellular processes and responses.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-ERK 1/2 Antibody (C-9) has 542 citations in a variety of scientific publications.

Primary antibodies like Anti-ERK 1/2 Antibody (C-9) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

ERK 1/2 Antibody (C-9) Specifications

Alternate Product Name: p44/42 MAPK

Product Clone Name: C-9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 101-172

Epitope Target: ERK 2

Epitope Species: human

Product Reactivity/Detection: ERK 1 and ERK 2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 542 citations in various scientific publications

Available Conjugates/Forms for ERK 1/2 Antibody (C-9): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred p-ERK antibody is p-ERK Antibody (E-4): sc-7383.