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- mouse monoclonal IgG2a; 200 µg/ml
- raised against amino acids 108-300 of E2F-4 of human origin
- recommended for detection of E2F-4 of mouse and human origin by WB, IP, IF and IHC(P)
- TransCruz reagent for ChIP application, sc-511 X, 200 µg/0.1 ml
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E2F-4 Background Information
The human retinoblastoma gene product appears to play an important role in the negative regulation of cell proliferation. Functional inactivation of Rb can be mediated either through mutation or as a consequence of interaction with DNA tumor virus-encoded proteins. Of all the Rb associations described to date, the identification of a complex between Rb and the transcription factor E2F most directly implicates Rb in regulation of cell proliferation. E2F was originally identified through its role in transcriptional activation of the adenovirus E2 promoter. Sequences homologous to the E2F binding site have been found upstream of a number of genes that encode proteins with putative functions in the G1 and S phases of the cell cycle. E2F-1 is a member of a broader family of transcription regulators including E2F-2, E2F-3, E2F-4, E2F-5, E2F-6 and E2F-7 each of which forms heterodimers with a second protein, DP-1, forming an “active” E2F transcriptional regulatory complex.
| E2F-4 (RK-13) Product Citations |
See how others have used E2F-4 (RK-13): sc-511 antibody and or E2F-4 (RK-13) antibody conjugates.
8 total citations Loading citations.
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E2F-4 (RK-13)
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E2F-4 (RK-13): sc-511. Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear staining.
Western blot analysis of human recombinant E2F-4 (108-300): sc-4098 WB (A,C) and unrelated control (B,D) fusion proteins. Antibodies tested include E2F-4 (C-108): sc-512 (A,B) and E2F-4 (RK-13): sc-511 (C,D). ChIP analysis of c-Myc promoter occupancy in response to lithium stimulation in serum synchronized C2C12 cells. Antibodies tested include HDAC1 (H-15): sc-7872, HDAC1 (C-19): sc-6298 and HDAC1 (H-11): sc-8410 (A), β-catenin (H-120): sc-7199, β-catenin (C-18): sc-1496 and β-catenin (E-5): sc-7963 (B), E2F-4 (A-20): sc-1082, E2F-4 (D-3): sc-6851, E2F-4 (RK-13): sc-511 and E2F-4 (C-20): sc-866 (C), and p130 (C-20): sc-317, p130 (211.6): sc-9963 and p130 (H-125): sc-20678 (D). Data kindly provided by M.G. Rosenfeld and reproduced with permission from Proc. Natl. Acad. Sci. 100: 3245-3250. Copyright 2003 National Academy of Sciences, USA. E2F-4 (RK-13): sc-511. Immunoperoxidase staining of formalin fixed, paraffin-embedded human fallopian tube tissue showing cytoplasmic and membrane staining of glandular cells (low and high magnification). Kindly provided by The Swedish Human Protein Atlas (HPA) program.
E2F-4 (RK-13): sc-511. Western blot analysis of E2F-4 expression in non-transfected: sc-117752 (A) and mouse E2F-4 transfected: sc-119883 (B) 293T whole cell lysates. E2F-4 (RK-13): sc-511. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human pancreas tumor.
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