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- mouse monoclonal IgG1; 200 µg/ml
- raised against a synthetic peptide corresponding to amino acids 41-54 of human Bcl-2
- recommended for detection of Bcl-2 of human origin by WB, IP, IF, IHC(P) and FCM
- agarose conjugate for IP studies, sc-509 AC, 500 µg/0.25 ml agarose
- HRP conjugate, sc-509 HRP, 200 µg/ml
- phycoerythrin (sc-509 PE), PerCP (sc-509 PerCP) and PerCP-Cy5.5 (sc-509 PCPC5) conjugates for intracellular FCM, 100 tests
- fluorescein (sc-509 FITC) and rhodamine (sc-509 TRITC) conjugates for immunofluorescence, 200 µg/ml
- Alexa Fluor® 405 (sc-509 AF405), Alexa Fluor® 488 (sc-509 AF488) and Alexa Fluor® 647 (sc-509 AF647) conjugates for FCM or immunofluorescence; 100 µg/2 ml
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Bcl-2 Background Information
Apoptosis is defined as a set of cascades which, when initiated, programs the cell to undergo lethal changes such as membrane blebbing, mitochondrial break down and DNA fragmentation. Bcl-2 is one among many key regulators of apoptosis, which are essential for proper development, tissue homeostasis, and protection against foreign pathogens. Human Bcl-2 is an anti-apoptotic, membrane-associated oncoprotein that can promote cell survival through protein-protein interactions with other Bcl-2 related family members, such as the death suppressors Bcl-xl, Mcl-1, Bcl-w, and A1 or the death agonists Bax, Bak, Bik, Bad, and Bid. The anti-apoptotic function of Bcl-2 can also be regulated through proteolytic processing and phospho-rylation. Bcl-2 may promote cell survival by interfering with the activation of the cytochrome c/Apaf-1 pathway through stabilization of the mitochondrial membrane. Mutations in the Bcl-2 gene can contribute to cancers where normal physiological cell death mechanisms are compromised by deregulation of the anti-apoptotic influence of Bcl-2.
| Bcl-2 (100) Product Citations |
See how others have used Bcl-2 (100): sc-509 antibody and or Bcl-2 (100) antibody conjugates.
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Bcl-2 (100)
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Western blot analysis of Bcl-2 expression in U-937 (A,D), HL-60 (B,E) and Jurkat (C,F) whole cell lysates. Antibodies tested include Bcl-2 (100): sc-509 (A-C) and Bcl-2 (N-19): sc-492 (D-F). Bcl-2 (100): sc-509. Immunoperoxidase staining of formalin-fixed, paraffin-embedded normal human colon tissue (A) showing cytoplasmic staining of lymphoid cells. Immunofluorescence staining of methanol-fixed HL-60 cells showing cytoplasmic staining (B).
Western blot analysis of Bcl-2 expression in HL-60 (A,D), Jurkat (B,E) and WEHI-231 (C,F) whole cell lysates. Antibodies tested include Bcl-2 (C-2): sc-7382 (A-C) and Bcl-2 (100): sc-509 (D-F). Bcl-2 (100) PE: sc-509 PE. Intracellular FCM analysis of fixed and permeabilized Jurkat cells. Black line histogram represents the isotype control, normal mouse IgG 1: sc-2866. Bcl-2 (100) Alexa Fluor 488: sc-509 AF488. Intracellular FCM analysis of fixed and permeabilized Jurkat cells. Black line histogram represents the isotype control, normal mouse IgG 1: sc-3890. Bcl-2 (100) Alexa Fluor 647: sc-509 AF647. Intracellular FCM analysis of fixed and permeabilized Jurkat cells. Black line histogram represents the isotype control, normal mouse IgG 1: sc-24636. Bcl-2 (100) Alexa Fluor 488: sc-509 AF488. Immunofluorescence staining of methanol-fixed HL-60 cells showing cytoplasmic localization using indirect Texas Red (A) staining and HeLa cells using direct Alexa Fluor 488 (B) staining.
Bcl-2 (100): sc-509. Western blot analysis of Bcl-2 expression in non-transfected: sc-117752 (A) and human Bcl-2 transfected: sc-176463 (B) 293T whole cell lysates. Bcl-2 (100) FITC: sc-509 FITC. Intracellular FCM analysis of fixed and permeabilized Jurkat cells. Black line histogram represents the isotype control, mouse IgG 1: sc-2855. goat anti-mouse IgG 1-HRP: sc-2969. Western blot analysis of Bcl-2 expression in Jurkat whole cell lysate. Antibody tested: Bcl-2 (100): sc-509. donkey anti-mouse IgG-HRP: sc-2314. Western blot analysis of Bcl-2 expression in Jurkat whole cell lysate. Antibody tested (IgG 1): Bcl-2 (100): sc-509. |
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