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eIF4AI/II Antibody (H-145): sc-50354

 |  Datasheet

(Based on data analysis)

  • eIF4AI/II Antibody (H-145) is a rabbit polyclonal IgG provided at 200 µg/ml
  • epitope corresponding to amino acids 186-330 mapping within an internal region of eIF4AI of human origin
  • recommended for detection of eIF4AI and eIF4AII of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including equine, canine, bovine, porcine and avian
 
Full-length eIF4AI/II protein sequence with immunogen highlighted:
MSASQDSRSRDNGPDGMEPEGVIESNWNEIVDSFDDMNLSESLLRGIYAYGFEKPSAIQQRAILPCIKGYDVIAQAQSGTGKTATFAISILQQIELDLKATQALVLAPTRELAQQIQKVVMALGDYMGASCHACIGGTNVRAEVQKLQMEAPHIIVGTPGRVFDMLNRRYLSPKYIKMFVLDEADEMLSRGFKDQIYDIFQKLNSNTQVVLLSATMPSDVLEVTKKFMRDPIRILVKKEELTLEGIRQFYINVEREEWKLDTLCDLYETLTITQAVIFINTRRKVDWLTEKMHARDFTVSAMHGDMDQKERDVIMREFRSGSSRVLITTDLLARGIDVQQVSLVINYDLPTNRENYIHRIGRGGRFGRKGVAINMVTEEDKRTLRDIETFYNTSIEEMPLNVADLI

See additional eIF Antibodies including eIF, eIF1, eIF1/1B, eIF1AD, eIF1AX, eIF1AY, eIF2 alpha, eIF2 beta, eIF2B alpha, eIF2B beta, eIF2B delta, eIF2B epsilon, eIF2B gamma, eIF2C, eIF2D, eIF3 alpha, eIF3 beta, eIF3 delta, eIF3 epsilon, eIF3 eta, eIF3 gamma, eIF3 theta, eIF3 zeta, eIF3e, eIF3K, eIF3M, eIF4A, eIF4AI, eIF4AII, eIF4AIII, eIF4B, eIF4E, eIF4G, eIF4GII, eIF4H, eIF5, eIF5A, eIF5B, eIF6, eIF2C1, eIF2C2, eIF2C3, eIF2S3, eIF2C4, eIF2C5, EIF3S6IP, eIF4E2, eIF4E3 and eIF3 p110.

Also see eIF Inhibitors for functional analysis of cellular responses to eIF.


Ordering InformationGene Info
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 Ordering Information
Product NameCatalog #UnitPriceQtyAdd 
eIF4AI/II Antibody (H-145) sc-50354 200 µg/ml $279
 WB Positive Control Cell Lysates (click product name for more information)
Product NameCatalog #UnitPriceQtyAdd 
eIF4AII (h2): 293 Lysate sc-113335 100 µg/200 µl $205
eIF4AI (m): 293T Lysate sc-125292 100 µg/200 µl $205
HeLa Whole Cell Lysate sc-2200 500 µg/200 µl $104
mouse brain extract sc-2253 500 µg/200 µl $104
eIF4AII (h2): 293T Lysate sc-173528 100 µg/200 µl $205
K-562 Whole Cell Lysate sc-2203 500 µg/200 µl $104
 
Species Gene Name Gene ID Chromosome Location Isoform (mRNA) Accession # Protein Accession # OMIM™ Number
Human EIF4A1 1973 17p13.1 NM_001416 P60842
 602641
Human EIF4A2 1974 3q27.3 NM_001967 Q14240
 601102
Mouse Eif4a1 13681 11 B3 P60843
 N/A
Mouse Eif4a2 13682 16 B1 P10630
 N/A
 

Translation initiation in eukaryotes necessitates the assembly of an 80S ribosomal complex. Eukaryotic initiation factors (eIFs) are utilized in a sequence of reactions that leads to 80S ribosomal assembly and initiation of translation. Mammalian eukaryotic translation initiation factor 4F (eIF4F) is a protein complex that contains eIF4A, eIF4E and eIF4G, binds mRNA at a 5’-cap motif and recruits the 43S ribosomal preinitiation complex to the eligible transcript. Along with eIF4B, the eIF4F complex mediates the unwinding of mRNA secondary structure to facilitate ribosome association. eIF4E specifically interacts with the 5’ cap, eIF4A(I,II) are bidirectional RNA helicases, and eIF4G(I,II) are scaffolding proteins which coordinate eIF4E, eIF4A, eIF3 and the 40S ribosome. Human eIF4AI (eIF4A, DDX2A) is a 406 amino acid protein that is 92.7% homologous to mouse eIF4AI. The promoter region of human eIF4A1 contains TATA and CAAT motifs and consensus binding sites to Sp1 and AP2.

eIF4AI/II Antibody (H-145) Data
Click on image to enlarge
sc-50354
eIF4AI/II (H-145): sc-50354. Western blot analysis of eIF4AI/II expression in HeLa whole cell lysate.
sc-50354
eIF4AI/II (H-145): sc-50354. Western blot analysis of eIF4AII expression in non-transfected 293: sc-110760 (A), human eIF4AII transfected 293: sc-113335 (B) and HeLa (C) whole cell lysates.
sc-50354
eIF4AI/II (H-145): sc-50354. Western blot analysis of eIF4AI and eIF4AII expression in non-transfected: sc-117752 (A) and human eIF4AII transfected: sc-113335 (B) 293T whole cell lysates and mouse brain tissue extract (C).
sc-50354
eIF4AI/II (H-145): sc-50354. Western blot analysis of eIF4AI expression in non-transfected 293T: sc-117752 (A), mouse eIF4AI transfected 293T: sc-125292 (B) and HeLa (C) whole cell lysates.
sc-50354
eIF4AI/II (H-145): sc-50354. Western blot analysis of eIF4AI/II expression in mouse brain tissue extract.
sc-50354
eIF4AI/II (H-145): sc-50354. Western blot analysis of eIF4AII expression in non-transfected 293T: sc-117752 (A), human eIF4AII transfected 293T: sc-173528 (B) and HeLa (C) whole cell lysates.
sc-50354
eIF4AI/II (H-145): sc-50354. Western blot analysis of eIF4AI/II expression in K-562 whole cell lysate.
sc-50354
eIF4AI/II (H-145): sc-50354. Immunoperoxidase staining of formalin fixed, paraffin-embedded human placenta tissue showing cytoplasmic staining of trophoblastic cells.
sc-50354
eIF4AI/II (H-145): sc-50354. Immunoperoxidase staining of formalin fixed, paraffin-embedded human placenta tissue showing cytoplasmic staining of decidual cells.
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