epitope mapping at the N-terminus of Aprataxin of human origin
recommended for detection of Aprataxin isoforms 1, 3 and 8 of mouse, human and, to a lesser extent, rat origin by WB, IP, IF and ELISA; also reactive with additional species, including equine, bovine and porcine
TransCruz reagent for Gel Supershift and ChIP applications, sc-48842 X, 200 µg/0.1 ml
Aprataxin Background Information Aprataxin is a nuclear protein, present in both the nucleoplasm and the nucleolus, which is a member of the histidine triad (HIT) superfamily. Aprataxin is involved in DNA single-strand break repair, mediating protein-protein interactions with molecules responding to DNA damage. Aprataxin contains three conserved domains: an N-terminal forkhead-associated (FHA) domain which mediates protein-protein interactions, a HIT domain that is similar to Hint, and a C-terminal zinc finger domain. Loss of function mutations in APTX, the gene encoding for Aprataxin, destabilize the Aprataxin protein and result in a rare neurological disorder known as ataxia-oculomotor apraxia, characterized by abnormal movements of the head and eyes. These mutations either target the HIT domain or truncate the protein N-terminal to a zinc finger.
