epitope corresponding to amino acids 15-243 mapping within an internal region of FIH-1 of human origin
recommended for detection of FIH-1 of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including equine, bovine and porcine
FIH-1 Background Information Factor inhibiting HIF-1 (FIH-1) exists as a homodimer and binds to HIF-1å. Specifically, FIH-1 operates as an asparaginyl hydroxylase. It catalyzes the hydroxylation of the ∫-carbon of Asparagine residue 803 within the carboxy terminal transactivation domain of HIF-1å. This hydroxylation event blocks the association of HIF-1å with co-activators. FIH-1 also binds to von Hippel-Lindau (VHL) tumor suppressor protein, which represses transcriptional ac-tivity of HIF-1å. In transiently transfected human osteosarcoma cells, FIH-1 localizes to the cytoplasm. The structure of FIH-1 includes a jellyroll-like ∫-barrel containing ferrous-binding triad residues. The gene encoding human FIH-1 maps to chromosome 10q24.
FIH-1 (H-229) Product Citations
See how others have used FIH-1 (H-229): sc-48813 antibody and or FIH-1 (H-229) antibody conjugates.
