 |
- rabbit polyclonal IgG, 200 µg/ml
- epitope mapping at the C-terminus of Id1 of mouse origin
- recommended for detection of Id1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including canine, bovine and porcine
- blocking peptide, sc-488 P
- TransCruz reagent for Gel Supershift and ChIP applications, sc-488 X, 200 µg/0.1 ml
|
|
| |
Ordering InformationProduct Citations
Recommended Support Products:
(click button of application of choice)
Id1 Background Information
Members of the Id family of basic helix-loop-helix (bHLH) proteins include Id1, Id2, Id3 and Id4. They are ubiquitously expressed and dimerize with members of the class A and B HLH proteins. Due to the absence of the basic region, the resulting heterodimers cannot bind DNA. The Id-type proteins thus appear to negatively regulate DNA binding of bHLH proteins. Since Id1 inhibits DNA binding of E12 and Myo D, it apparently functions to inhibit muscle-specific gene expression. Under conditions that facilitate muscle cell differentiation, the Id protein levels fall, allowing E12 and/or E47 to form heterodimers with Myo D and myogenin, which in turn activate myogenic differentiation. It has been shown that expression of each of the Id proteins is strongly dependent on growth factor activation and that reduction of Id mRNA levels by antisense oligonucleotides leads to a delayed reentry of arrested cells into the cell cycle following growth factor stimulation.
| Id1 (C-20) Product Citations |
See how others have used Id1 (C-20): sc-488 antibody and or Id1 (C-20) antibody conjugates.
67 total citations Loading citations.
|
| |
|
|
|
Id1 (C-20)
Click on image to enlarge
|
|
Id1 (C-20): sc-488. Western blot analysis of Id1 expression in HeLa nuclear extract.
Western blot analysis of human recombinant Id1 (A,C) and Id1 His-tagged mouse recombinant Id1 (B,D) fusion proteins. Antibodies tested include Id1 (Z-8): sc-427 (A,B) and Id1 (C-20): sc-488 (C,D). Id1 (C-20): sc-488. Western blot analysis of Id1 expression in non-transfected: sc-110760 (A) and human Id1 transfected: sc-113028 (B) 293 whole cell lysates. Id1 (C-20): sc-488. Western blot analysis of Id1 expression in non-transfected: sc-117752 (A) and human Id1 transfected: sc-171632 (B) 293T whole cell lysates. Id1 (C-20): sc-488. Western blot analysis of Id1 expression in non-transfected: sc-117752 (A) and human Id1 transfected: sc-171709 (B) 293T whole cell lysates. Id1 (C-20): sc-488. Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.
Id1 (C-20): sc-488. Immunoperoxidase staining of formalin fixed, paraffin-embedded human bronchus tissue showing nuclear and cytoplasmic staining of respiratory epithelial cells.
|
|
Download
|
|
