epitope corresponding to amino acids 454-753 mapping at the C-terminus of MTF-1 of human origin
recommended for detection of MTF-1 of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including equine, canine, bovine and porcine
TransCruz reagent for Gel Supershift and ChIP applications, sc-48775 X, 200 µg/0.1 ml
MTF-1 Background Information The metal-responsive element (MRE)-binding transcription factor (MTF-1) stimulates the expression of metallothioneins in response to the exposure of cells to heavy metals (1-3). MTF-1 contains six zinc fingers in the DNA binding domain (2-3). The phosphorylation of MTF-1 in response to metal exposure appears to play a significant role in the ability of MTF-1 to activate metallothionein transcription (4). In addition to its role in metallothionein activation, MTF-1 is involved in a post-transcription regulatory complex for ribosomal protein S25 (5). MTF-1, La and p53 inhibit the nuclear export of S25 mRNA in response to nutrient depravation (5). Furthermore, MTF-1 acts as a chromatin insulator on integrated transgenes in cultured cells to insulate active loci against chromatin silencing (6).
MTF-1 (H-300)
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MTF-1 (H-300): sc-48775. Western blot analysis of MTF-1 expression in non-transfected: sc-117752 (A) and mouse MTF-1 transfected: sc-125651 (B) 293T whole cell lysates and mouse heart tissue extract (C).
MTF-1 (H-300): sc-48775. Western blot analysis of MTF-1 expression in non transfected (A) and human MTF-1 transfected (B) HEK293 whole cell lysates.
