epitope corresponding to amino acids 1-300 mapping at the N-terminus of HIRA of human origin
recommended for detection of HIRA of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including canine and avian
HIRA Background Information HIRA is the human homolog of yeast Hir1p and Hir2p and is a widely expressed nuclear protein involved in cell cycle regulation. Specifically, HIRA is essential during development, possibly through the control of
specific gene transcription programs. During development, HIRA is highly expressed in regions that contain neural crest cells. Cyclin dependent kinase 2 (Cdk-2) and Cyclin A bind with HIRA at an RXL motif which results in phosphorylation of the substrate at Thr 555. Ectopic expression of HIRA results in cell cycle arrest in S phase. HIRA also contains seven copies of a WD repeat and exhibits histone binding properties, suggesting that it may function as a regulator of histone gene expression. The gene encoding the 1017 amino acid HIRA maps to human chromosome 22q11, an area known to be the critical region of DiGeorge Syndrome (DGS). DGS is a congenital disease characterized by defects in tissues and organs, whose development depends on cell populations derived from the neural crest.
HIRA (H-300)
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HIRA (H-300): sc-48774. Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear and cytoplasmic localization.
