PIF1 Background Information PIF1 is a single-stranded (ss) DNA-dependent ATPase as well as a DNA helicase that unwinds DNA in a 5' to 3' direction with respect to the DNA strand on which it binds first. This protein is critical to DNA replication and telomere length maintenance in Saccharomyces cerevisiae. The PIF1 gene is highly conserved from yeast to humans. Mutations in the gene that encodes for PIF1 cause all telomeres to lengthen, suggesting that the PIF1 functions as a catalytic inhibitor of both new telomere formation and telomere elongation. Human PIF1 preferentially binds telomeric DNA where it inhibits telomerase activity. PIF1 specifically counteracts the RecQ homolog Sgs1 helicase activity, and degradation of PIF1 is mediated by the ubiquitin-26S proteasome pathway.
PIF1 (F-10)
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PIF1 (F-10): sc-48377. Western blot analysis of PIF1 expression in HeLa (A), K-562 (B), NIH/3T3 (C) and KNRK (D) nuclear extracts.
PIF1 (F-10): sc-48377. Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.
PIF1 (F-10): sc-48377. Immunoperoxidase staining of formalin fixed, paraffin-embedded human appendix tissue showing nuclear staining of glandular and lymphoid cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
PIF1 (F-10): sc-48377. Western blot analysis of PIF1 expression in non-transfected 293T: sc-117752 (A), mouse PIF1 transfected 293T: sc-122568 (B) and K-562 (C) whole cell lysates.
