epitope mapping at the N-terminus of Mds1 of human origin
recommended for detection of Mds1 and Mds1-Evi-1 fusion protein of human origin by WB, IF and ELISA; also reactive with additional species, including equine and porcine
TransCruz reagent for Gel Supershift and ChIP applications, sc-48274 X, 200 µg/0.1 ml
Evi-1 Background Information The Mds1 and Evi-1 genes located on human chromosome 3q26.2 form a complex locus that encodes three different proteins: Mds1, Evi-1 and a Mds1-Evi-1 fusion protein. Mds1 is a 169 amino acid protein that has lower expression levels than either Mds1-Evi-1 fusion protein or Evi-1. The Mds1-Evi-1 fusion protein is expressed in both normal and leukemic tissues and contains several zinc finger domains. Evi-1 contains two zinc finger domains, the second of which is essential for transactivation of the c-Fos promoter and for AP-1 activation. The first zinc finger domain in Evi-1 binds to Smad3, suppressing its activity and inhibiting TGF∫ signaling. The t(3;21) (q26;q22) chromosomal translocation of Evi-1 produces a chimeric transcription factor, AML-1/Evi-1, that appears to suppress the transactivation of AML-1, which is a stimulator of myeloid cell differentiation. Inappropriate Evi-1 gene expression in hemato-poietic cells has been shown to be associated with acute myelogenous leukemia (AML) and myelodysplastic syndromes.
