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- goat polyclonal IgG, 200µg/ml
- epitope mapping near the N-terminus of BCAR3 of human origin
- recommended for detection of BCAR3 of mouse and human origin by WB, IP, IF and ELISA; also reactive with additional species, including equine, canine, bovine and porcine
- blocking peptide, sc-47811 P
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Ordering Information
Recommended Support Products:
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| Species |
Gene Name |
Gene ID |
Chromosome Location |
Isoform (mRNA) Accession # |
Protein Accession # |
OMIM™ Number |
| Human |
BCAR3 |
8412 |
1p22.1 |
NM_003567 |
O75815
|
604704 |
| Mouse |
Bcar3 |
29815 |
3 G1 |
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Q9QZK2
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N/A |
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BCAR3 Background Information
BCAR3 (breast cancer anti-estrogen resistance 3, also designated AND-34 in murine; a novel SH2-containing protein 2 or NSP2, and SH2D3B) overexpression is a characteristic of antiestrogen resistance in human ZR-75-1 breast cancer cells. The deduced 825-amino acid BCAR3 protein contains a Src homology 2 (SH2) domain and shares homology to yeast CDC48. A 3.4-kb BCAR3 transcript is present in heart, placenta, skeletal muscle, spleen, prostate, testis, ovary, small intestine, colon, fetal kidney, and tamoxifen-resistant cancer cell lines. BCAR3 acts as a regulator of R-Ras to mediate the level of insulin receptor substrate 1 (IRS-1) in MCF-7 and ZR-75-1 breast cancer cell lines. BCAR3 also interacts with p130Cas to enhance Src activation and cell migration. |
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BCAR3 (N-15)
Click on image to enlarge
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BCAR3 (N-15): sc-47811. Western blot analysis of BCAR3 expression in SK-BR-3 (A), BT-20 (B) and MCF7 (C) whole cell lysates.
BCAR3 (N-15): sc-47811. Western blot analysis of BCAR3 expression in MCF7 whole cell lysate.
BCAR3 (N-15): sc-47811. Western blot analysis of BCAR3 expression in non-transfected: sc-117752 (A) and human BCAR3 transfected: sc-115467 (B) 293T whole cell lysates. BCAR3 (N-15): sc-47811. Immunofluorescence staining of methanol-fixed HeLa cells showing membrane and cytoplasmic localization.
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