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β-Actin Antibody (C4): sc-47778

 |  Datasheet

(Based on data analysis)

  • β-Actin Antibody (C4) is a mouse monoclonal IgG1 provided at 200 µg/ml
  • raised against gizzard Actin of avian origin
  • recommended for detection of β-Actin of mouse, rat, human, avian, bovine, canine, porcine, rabbit, Dictyostelium discoideum and Physarum polycephalum origin by WB, IP, IF, IHC(P) and ELISA; may cross-react with all six known isoforms of Actin in higher vertebrates (including cytoplasmic β- and γ- Actin isoforms, skeletal, cardiac, and vascular α-Actin isoforms, and enteric γ-Actin isoform)
  • agarose conjugate for IP studies, β-Actin (C4) AC: sc-47778 AC, 500 µg/0.25 ml agarose
  • HRP conjugate, β-Actin (C4) HRP: sc-47778 HRP, 200 µg/ml
  • biotin conjugate, β-Actin (C4) B: sc-47778 B, 200 µg/1 ml
  • TransCruz reagent for Gel Supershift and ChIP applications, sc-47778 X, 200 µg/0.1 ml
  • See 565 citations for β-Actin Antibody (C4)
 

See additional Actin Antibodies including Actin, alpha-Actin, beta-Actin, gamma-Actin, kappa-Actin, Muscle Actin and Smooth Muscle Actin.

Also see chemical Actin Inhibitors for functional analysis of cellular responses to Actin.


Ordering InformationProduct CitationsGene Info
Recommended Support Products:
(click button of application of choice)
WB   IP   IF   IHC(P)   siRNA  
Set Currency

 Ordering Information
Product NameCatalog #UnitPriceQtyAddFavorites
β-Actin Antibody (C4) sc-47778 200 µg/ml $279
β-Actin Antibody (C4) AC sc-47778 AC 500 µg/ml, 25% ag $366
β-Actin Antibody (C4) B sc-47778 B 200 µg/ml $282
β-Actin Antibody (C4) HRP sc-47778 HRP 200 µg/ml $279
β-Actin Antibody (C4) X sc-47778 X 200 µg/0.1 ml $279
 CRISPR/Cas9 KO and HDR Plasmids (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
β-Actin CRISPR/Cas9 KO Plasmid (h) sc-400000 20 µg $345
β-Actin HDR Plasmid (h) sc-400000-HDR 20 µg $385
β-actin CRISPR/Cas9 KO Plasmid (m) sc-418965 20 µg $345
β-actin HDR Plasmid (m) sc-418965-HDR 20 µg $385
 siRNA Gene Silencers (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
β-Actin siRNA (h) sc-108069 10 µM $258
β-Actin siRNA (m) sc-108070 10 µM $258
β-Actin siRNA (r) sc-156106 10 µM $258
β-Actin (h)-PR sc-108069-PR 10 µM, 20 µl $23
β-Actin (m)-PR sc-108070-PR 10 µM, 20 µl $23
β-Actin (r)-PR sc-156106-PR 10 µM, 20 µl $23
 shRNA Plasmids (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
β-Actin shRNA Plasmid (h) sc-108069-SH 20 µg $520
β-Actin shRNA Plasmid (m) sc-108070-SH 20 µg $520
β-Actin shRNA Plasmid (r) sc-156106-SH 20 µg $520
 shRNA Lentiviral Particles (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
β-Actin shRNA (h) Lentiviral Particles sc-108069-V 200 µl $625
β-Actin shRNA (m) Lentiviral Particles sc-108070-V 200 µl $625
β-Actin shRNA (r) Lentiviral Particles sc-156106-V 200 µl $625
 WB Positive Control Cell Lysates (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
C32 Whole Cell Lysate sc-2205 500 µg/200 µl $104
BC3H1 Cell Lysate sc-2299 500 µg/200 µl $104
HeLa Whole Cell Lysate sc-2200 500 µg/200 µl $104
γ-Actin (h): 293T Lysate sc-116428 100 µg/200 µl $205
ACTG2 (m): 293T Lysate sc-118229 100 µg/200 µl $205
 
Species Gene Name Gene ID Chromosome Location Isoform (mRNA) Accession # Protein Accession # OMIM™ Number
Human ACTB 60 7p22.1 NM_001101 P60709
102630
Mouse Actb 11461 5 G2 P60710
N/A
 


All eukaryotic cells express Actin, which often constitutes as much as 50% of total cellular protein. Actin filaments can form both stable and labile structures and are crucial components of microvilli and the contractile apparatus of muscle cells. While lower eukaryotes, such as yeast, have only one Actin gene, higher eukaryotes have several isoforms encoded by a family of genes. At least six types of Actin are present in mammalian tissues and fall into three classes. å-Actin expression is limited to various types of muscle, whereas -∫ and ©-Actin are the principle constituents of filaments in other tissues. Members of the small GTPase family regulate the organization of the Actin cytoskeleton. Rho controls the assembly of Actin stress fibers and focal adhesion. Rac regulates Actin filament accumulation at the plasma membrane. Cdc42 stimulates formation of filopodia.

β-Actin Antibody (C4) Product Citations
See how others have used β-Actin Antibody (C4) and or β-Actin Antibody (C4) conjugates.


565 total citations
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β-Actin Antibody (C4) Data
Click on image to enlarge
β-Actin (C4): sc-47778. Western blot analysis of β-Actin expression in A-10 whole cell lysate.
β-Actin (C4): sc-47778. Western blot analysis of β-Actin expression in HeLa (A), Sol8 (B), C32 (C), NIH/3T3 (D) and KNRK (E) whole cell lysates
β-Actin (C4): sc-47778. Western blot analysis of β-Actin expression in C32 (A), BC3H1 (B), KNRK (C), HeLa (D), NIH/3T3 (E) and IMR-32 (F) whole cell lysates.
β-Actin (C4): sc-47778. Immunoperoxidase staining of formalin fixed, paraffin-embedded human spleen tissue showing cytoskeletal staining of cells in red and white pulps.
β-Actin (C4): sc-47778. Western blot analysis of ACTA1 expression in non-transfected 293T: sc-117752 (A), mouse ACTA1 transfected 293T: sc-118228 (B) and C32 (C) whole cell lysates.
β-Actin (C4): sc-47778. Western blot analysis of ACTG2 expression in non-transfected: sc-117752 (A) and mouse ACTG2 transfected: sc-118229 (B) 293T whole cell lysates.
β-Actin (C4): sc-47778. Western blot analysis of γ-Actin expression in non-transfected 293T: sc-117752 (A), human γ-Actin transfected 293T: sc-116428 (B) and HeLa (C) whole cell lysates.
β-Actin (C4): sc-47778. Western blot analysis of ACTG1 expression in non-transfected 293T: sc-117752 (A), mouse ACTG1 transfected 293T: sc-124922 (B), HeLa (C) and A-431(D) whole cell lysates.
β-Actin (C4): sc-47778. Western blot analysis of β-Actin expression in non-transfected 293T: sc-117752 (A), human β-Actin transfected 293T: sc-111134 (B) and C32 (C) whole cell lysates.
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