TRF2 Background Information Telomeric repeat binding factor 1 (TERF1, PIN2, TRF1, TRBF1) and telomeric repeat binding factor 2 (TERF2, TRF2, TRBF2) are present at telomeres throughout the cell cycle, where they regulate telomerase by acting in cis to limit the elongation of individual chromosome ends. Telomerase adds hexameric repeats of 5'-TTAGGG-3' to the ends of chromosomal DNA. This telomerase enzyme plays an influential role in cellular immortalization and cellular senescence. TRF1 negatively regulates telomere elongation, while TRF2 protects the chromosome ends by inhibiting end-to-end fusions. Down-regulation of TRF expression in tumor cells may contribute to cell immortalization and malignant progression. TRF1 has an acidic N-terminus while TRF2 has a basic N-terminus. TRF2 localizes in the nucleolus at G0 and S and diffuses out of the nucleolus in G2 phase. During mitosis TRF2 disperses from the condensed chromosomes and returns to the nucleolus at cytokinesis.
TRF2 (9F10)
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TRF2 (9F10): sc-47693. Western blot analysis of TRF2 expression in HeLa (A), Jurkat (B) and K-562 (C) whole cell lysates.
TRF2 (9F10): sc-47693. Western blot analysis of TRF2 expression in non-transfected: sc-117752 (A) and human TRF2 transfected: sc-113675 (B) 293T whole cell lysates and K-562 nuclear extract (C).
TRF2 (9F10): sc-47693. Western blot analysis of TRF2 expression in non-transfected 293T: sc-117752 (A), human TRF2 transfected 293T: sc-113675 (B) and HeLa (C) whole cell lysates.
