epitope mapping at the N-terminus of PARN of human origin
recommended for detection of PARN of mouse, rat and human origin by WB, IF and ELISA; also reactive with additional species, including canine and bovine
TransCruz reagent for Gel Supershift and ChIP applications, sc-47619 X, 200 µg/0.1 ml
PARN Background Information Exonucleolytic degradation of the poly(A) tail often initiates the first step in the decay of eukaryotic mRNAs. Poly(A)-specific ribonuclease (PARN), a highly poly(A)-specific 3'-exoribonuclease, efficiently degrades mRNA poly(A) tails. PARN, which also may be designated deadenylating nuclease, may also be involved in nonsense-mediated mRNA decay, a critical process of selective degradation of mRNAs that contain premature stop codons, and in the degradation of inherently unstable mRNAs that contain au-rich elements (AREs) in their 3' untranslated regions. PARN, which can form a homodimer, interacts with KHSRP and can be found in a mRNA decay complex with RENT1, RENT2 and RENT3B.It localizes mainly to the nucleus (may be detected in the nucleolus), but may also localize to the cytoplasm.
