epitope mapping within an internal region of NEIL1 of human origin
recommended for detection of NEIL1, isoform 1 of mouse and human origin by WB, IF and ELISA; non cross-reactive with isoform 2; also reactive with additional species, including equine, canine, bovine and porcine
TransCruz reagent for Gel Supershift and ChIP applications, sc-47608 X, 200 µg/0.1 ml
NEIL1 Background Information NEIL1, NEIL2 and NEIL3, also known as Endonuclease VIII-like 1,2 and 3 or DNA-(apurinic or apyrimidinic site) lyase NEIL 1, 2 and 3, are nuclear proteins involved in the repair of DNA damaged by oxidation. The NEIL proteins belong to the FPG familiy of proteins. They act as DNA glycosylases that can recognize and remove damaged bases, leaving an abasic site. NEIL3, however, lacks the proline residue at the N-terminus which acts as the active site residue found in NEIL1 and NEIL2. NEIL1 is a ubiquitiously expressed protein that is up-regulated during S-phase. NEIL2 is expressed primarily in testis, heart, skeletal muscle, placenta, brain, kidney and liver while NEIL3 is detected primarily in thymus and testis.
