epitope mapping near the N-terminus of Uev1A of human origin
recommended for detection of Uev1A isoform 1 and Kua of human origin by WB, IF and ELISA; also reactive with additional species, including equine, canine and avian
Uev1A Background Information Uev1A, also designated Uev1, UBE2V1 for Ubiquitin-conjugating enzyme E2 variant 1 and CROC1, shows sequence similarity to other ubiquitin-conjugating enzymes, but lacks the conserved cysteine residue critical for their catalytic activity (1-3). Therefore, Uev1A does not have ubiquitin-conjugating activity in vitro (4). However, constitutive expression of exogenous Uev1A in colon carcinoma cells inhibits their capacity to differentiate upon confluence (4). Studies on recombinant Uev1A show that it localizes to the nucleus, excluding the nucleolar regions (1). Uev1A functions with TRAF6, a RING domain protein, to catalyze the synthesis of unique polyubiquitin chains linked through lysine-63 of ubiquitin (5). The gene encoding Uev1A maps to human chromosome 20q13.2 (4).
