epitope mapping within an internal region of GW182 of human origin
recommended for detection of GW182 isoforms 1, 2 and 3 only of mouse and human origin by WB, IP, IF and ELISA; also reactive with additional species, including canine, bovine and avian
GW182 Background Information GW bodies (GWBs) function as storage centers and degradation sites for mRNAs. GWBs are crucial intracellular structures for miRNA function. Dis-assembly or disruption of GWBs has been shown to impair siRNA and miRNA silencing activity. GW182 is a cytoplasmic marker protein for GWBs. GW182 autoantigen, also designated EMSY interactor protein, plays a role in the maintenance and stability of the GWB structures. GW182 is a ubiquitously expressed protein that binds to mRNA. The GW182 protein may interact with endogenous argonaute-2 (Ago2), which is also enriched in GWBs. The GW182 protein is detected in patients with ataxia, Sjoegren’s syndrome (SS) and sensor neuropathy disease, who develop autoantibodies against GWB structure proteins.
GW182 (M-17)
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GW182 (M-17): sc-47036. Western blot analysis of GW182 expression in non-transfected: sc-117752 (A) and human GW182 transfected: sc-113721 (B) 293T whole cell lysates.
