RPA194 Background Information RNA polymerases transcribe nuclear genes for ribosomal RNA, thus representing ribosomal biogenesis. RNA polymerase I (Pol I) is located in the nucleolus and transcribes class I genes, which code for large ribosomal RNA. Different subunits of the Pol I transcription machinery are targets of various physiological stimuli, which suggests that multiple signaling pathways are involved in carrying out Pol I transcription. RPA40 and RPA16 are subunits of Pol I that associate with each other at an early stage of RNA polymerase I assembly. RPA40 is essential for the function and integrity of the complex and is also an essential subunit of RNA polymerase III (Pol III). RPA40, RPA16 and RPA135 encode the three subunits of RNA polymerase I, respectively. RPA194 is the largest subunit of RNA Pol I and is not a component of Pol II and Pol III.
RPA194 (F-6)
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RPA194 (F-6): sc-46699. Western blot analysis of RPA194 expression in RAW 264.7 (A) and KNRK (B) nuclear extracts.
RPA194 (F-6): sc-46699. Western blot analysis of RPA194 expression in 293T (A) and HeLa (B) whole cell lysates.
RPA194 (F-6): sc-46699. Western blot analysis of RPA194 expression in non-transfected 293T: sc-117752 (A), truncated human RPA194 transfected 293T: sc-111445 (B) and KNRK (C) whole cell lysates.
RPA194 (F-6): sc-46699. Immunofluorescence staining of methanol-fixed HeLa cells showing nucleolar localization.
