epitope mapping near the C-terminus of TFIID p250 of human origin
recommended for detection of TFIID p250 of mouse, rat and human origin by WB, IF and ELISA; also reactive with additional species, including equine, canine and bovine
blocking peptide, sc-46648 P
TransCruz reagent for Gel Supershift and ChIP applications, sc-46648 X, 200 µg/0.1 ml
TAF II p250 Background Information TFIID is a general transcription factor which initiates pre-initiation complex assembly through direct interaction with the TATA promoter element. It is a multi-subunit complex consisting of a small TATA-binding polypeptide and other TBP-associated factors (TAFs). Although native TFIID can mediate both activator-independent (basal) and activator-dependent transcription in reconstituted systems, TBP can mediate only basal transcription. The largest subunit (TAF) of TFIID is a protein designated TAF II p250. Of interest, TAF II p250 has been cloned and shown to be identical to CCG1, a nuclear DNA-binding protein known to be important for cell cycle progression. This part of TAF II p250 may serve a specific function in activation of a subset of genes important for cell cycle progression.
