RPA 14 kDa subunit (N-20) Antibody: sc-46509

RPA 14 kDa subunit Background Information
The single-stranded-DNA-binding proteins (SSBs) are essential for DNA function in prokaryotic and eukaryotic cells, mitochondria, phages and viruses. Replication protein A (RPA), a highly conserved eukaryotic protein, is a heterotrimeric SSB that is composed of three subunits, designated RPA 14 kDa (also known as RPA3), RPA 32 kDa and RPA 70 kDa. Together, these subunits play an important role in DNA replication, recombination and repair. RPA is one of the major damage-recognition structures involved in the early stage of nucleotide excision repair and may play a role in telomere maintenance. The binding of human RPA (hRPA) to DNA involves molecular polarity, in which initial hRPA binding occurs on the 5’ side of a ssDNA substrate and then extends in the 3’ direction to create a stably bound hRPA. The RPA 14 kDa subunit localizes to the nucleus and is the smallest component of the RPA complex, functioning with the other subunits to regulate various aspects of DNA metabolism.