epitope mapping within an internal region of ESET of human origin
recommended for detection of ESET of mouse, rat and human origin by WB, IF and ELISA; also reactive with additional species, including equine, canine, bovine, porcine and avian
blocking peptide, sc-46112 P
TransCruz reagent for Gel Supershift and ChIP applications, sc-46112 X, 200 µg/0.1 ml
ESET Background Information ERG-associated protein with SET domain (ESET), also designated Histone H3-K9 methyltransferase 4 or SET domain bifurcated 1, is a nuclear protein belonging to the histone-lysine methyltransferase family and to the Suvar3-9 subfamily. It is a highly conserved protein of 150 amino acids that has been implicated in chromatin structure modulation. ESET is excluded from cell nucleoli and areas of condensed chromatin and can associate with the nonpericentromeric regions of chromatin. The gene encoding for this protein, SETDB1, maps to chromosome 1q21. ESET is a histone methyltransferase, methylating Lys-9 of histone H3 and mutations within the SETDB1 gene abolishes its methyltransferase activity. This methylation acts as a tag for epigenetic transcriptional repression by rounding up HP1 proteins to methylated histones. ESET is widely expressed with highest levels found in testis.
