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ERα siRNA (r), shRNA and Lentiviral Particle Gene Silencers

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See ER alpha Antibodies for analysis of cellular responses to ER alpha gene silencers, including ER alpha siRNA, ER alpha shRNA Plasmids and ER alpha shRNA Lentiviral Particles.

Also see ER alpha Inhibitors or ER alpha Activators for functional analysis of cellular responses to ER alpha.
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 Ordering Information
Product NameCatalog #UnitPriceQtyAdd 
ERα siRNA (r) sc-45949 10 µM $258
ERα (r)-PR sc-45949-PR 10 µM, 20 µl $23
ERα shRNA Plasmid (r) sc-45949-SH 20 µg $520
ERα shRNA (r) Lentiviral Particles sc-45949-V 200 µl $625

ERα siRNA (r): sc-45949

 |  siRNADatasheet / Protocol
  • 10 µM, 50-100 transfections
  • siRNA products generally consist of pools of three to five target-specific 19-25 nt siRNAs designed to knockdown gene expression
  • for independent verification of ERα gene silencing results, individual siRNA duplex components are also available upon request
  • suitable control antibody: ERα (MC-20): sc-542
  • sqRT-PCR Primer also available: ERα (r)-PR: sc-45949-PR, 20 µl
 
 Recommended siRNA Support Reagents (click product name for more information)
Product NameCatalog #UnitPriceQtyAdd 
siRNA Dilution Buffer sc-29527 1.5 ml $5
siRNA Transfection Reagent sc-29528 0.3 ml $142
siRNA Transfection Medium sc-36868 20 ml $9
siRNA Reagent System sc-45064 1 kit $331
Control siRNA-A sc-37007 10 µM $77
Control siRNA (FITC Conjugate)-A sc-36869 10 µM $104
ERα shRNA Plasmid (r): sc-45949-SH shRNA Datasheet / Protocol
  • 20 µg, up to 20 transfections
  • shRNA Plasmids generally consist of a pool of three to five lentiviral vector plasmids each encoding target-specific 19-25 nt (plus hairpin) shRNAs designed to knockdown gene expression
  • shRNA sequences correspond to ERα siRNA Gene Silencer sequences
  • provided as transfection-ready purified plasmid DNA target-specific gene silencing
  • after transfection, cells stably expressing shRNAs can be isolated by puromycin selection
  • shRNA Plasmids are intended only for non-commercial use. Click here for details.
  • suitable control antibody: ERα (MC-20): sc-542
  • sqRT-PCR Primer also available: ERα (r)-PR: sc-45949-PR, 20 µl
 
ERα shRNA (r)
Lentiviral Particles: sc-45949-V 		Lentiviral Particles Datasheet / Protocol
  • 200 µl viral stock containing 1x 106 infectious units of virus (IFU) , sufficient for 10-20 transductions
  • Lentiviral Particles are provided as transduction-ready viral particles for gene silencing
  • Lentiviral Particles generally contain three to five expression constructs each encoding target-specific 19-25 nt (plus hairpin) shRNA designed to knockdown gene expression
  • shRNA sequences correspond to ERα siRNA siRNA Gene Silencer sequences
  • After transduction, stable cell lines expressing the shRNA may be isolated via selection with puromycin
  • Biosafety - Lentiviral Particles are replication-incompetent and are designed to self-inactivate after transduction and integration of shRNA constructs into genomic DNA of target cells. Click here for details.
  • Lentiviral Particles are intended for non-commercial use. Click here for details.
 Recommended Lentiviral Particle Support Reagents (click product name for more information)
Product NameCatalog #UnitPriceQtyAdd 
Control shRNA Lentiviral Particles-A sc-108080 200 µl $176
copGFP Control Lentiviral Particles sc-108084 200 µl $176
Puromycin dihydrochloride sc-108071 25 mg $30
Polybrene® sc-134220 10 mg/ml $18
 Recommended Control Antibody (click product name for more information)
Product NameCatalog #UnitPriceQtyAdd 
ERα Antibody (MC-20) sc-542 200 µg/ml $279
goat anti-rabbit IgG-HRP sc-2004 200 µg/0.5 ml $70
 
Recommended Support Products:
(click button of application of choice)
 Recommended shRNA Support Reagents (click product name for more information)
Product NameCatalog #UnitPriceQtyAdd 
shRNA Plasmid Transfection Reagent sc-108061 0.2 ml $71
shRNA Plasmid Transfection Medium sc-108062 20 ml $8
Control shRNA Plasmid-A sc-108060 20 µg $83
Control shRNA Plasmid-B sc-108065 20 µg $83
Control shRNA Plasmid-C sc-108066 20 µg $83
copGFP Control Plasmid sc-108083 20 µg $83
Puromycin dihydrochloride sc-108071 25 mg $30