recommended for detection of glutathione-S-transferase (GST) of Schistosoma japonicum origin and GST fusion proteins
WB, IP, IF, FCM and ELISA of recombinant GST fusion proteins expressed in E. coli; designed to be used with GST expression vectors such as pGEX.3X and pGEX.2T
HRP conjugate, sc-459 HRP, 200 µg/ml
fluorescein conjugate for immunofluorescence, sc-459 FITC, 200 µg/ml
biotin conjugate, sc-459 B, 200 µg/ml
Alexa Fluor® 405 (sc-459 AF405), Alexa Fluor® 488 (sc-459 AF488) and Alexa Fluor® 647 (sc-459 AF647) conjugates for immunofluorescence; 100 µg/2 ml
GST Background Information Plasmid vectors for the expression of coding regions of eukaryotic genes in E. coli are in common usage; such expression vectors often encode hybrid fusion proteins containing part prokaryotic and part eukaryotic specified proteins. For instance, the pGEX.3X expression vector developed by Smith and Johnson allows for synthesis of fusion proteins between glutathionine-S-transferase (GST) and proteins encoded by inserted cDNA sequences. Antibodies derived from these GST fusion proteins are useful for checking protein expression both in plaques and on Western blots as well as for immunoaffinity purification of proteins expressed in E. coli.
GST (Z-5) Product Citations
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GST (Z-5)
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GST (Z-5): sc-459. Western blot analysis of human recombinant NFκB p50 (A) and GST (B) fusion proteins.
