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c-Jun (N) Antibody: sc-45

 |  Datasheet
  • rabbit polyclonal IgG, 200 µg/ml; also available as goat IgG, 200 µg/ml, sc-45-G
  • epitope mapping within the N-terminus of c-Jun of mouse origin
  • recommended for detection of c-Jun of mouse, rat, human, chicken and Xenopus origin by WB, IP, IF and ELISA; also reactive with additional species, including canine, bovine, porcine and avian
  • blocking peptide, sc-45 P
  • TransCruz reagent for Gel Supershift and ChIP applications, sc-45 X, 200 µg/0.1 ml
  • phycoerythrin conjugate for intracellular FCM, sc-45 PE, 100 tests
 
Additional c-Jun Antibodies ...
 
Ordering InformationProduct Citations
Recommended Support Products:
(click button of application of choice)
WB   IP   IF   Gel Shift   ChIP   siRNA  
 
Species Gene Name Gene ID Chromosome Location Isoform (mRNA) Accession # Protein Accession # OMIM™ Number
Human JUN 3725 1p32.1 NM_002228 P05412
165160
Human JUN 3725 1p32.1 P05412
165160
Mouse Jun 16476 4 C5 P05627
N/A
Mouse Jun 16476 4 C5 P05627
N/A
 
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 Ordering Information
Product NameCatalog #UnitPriceQtyAddFavorites
c-Jun (N) sc-45 200 µg/ml $279
c-Jun (N) P sc-45 P
(peptide)
100 µg/0.5 ml $61
c-Jun (N) PE sc-45 PE 100 tests in 2ml $303
c-Jun (N) X sc-45 X 200 µg/0.1 ml $279
c-Jun (N)-G sc-45-G 200 µg/ml $279
 siRNA Gene Silencers (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
c-Jun siRNA (h) sc-29223 10 µM $258
c-Jun siRNA (m) sc-29224 10 µM $258
c-Jun siRNA (h2) sc-44201 10 µM $258
c-Jun (h)-PR sc-29223-PR 10 µM $23
c-Jun (m)-PR sc-29224-PR 10 µM $23
c-Jun (h2)-PR sc-44201-PR 10 µM $23
 shRNA Plasmids (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
c-Jun shRNA Plasmid (h) sc-29223-SH 20 µg $520
c-Jun shRNA Plasmid (m) sc-29224-SH 20 µg $520
c-Jun shRNA Plasmid (h2) sc-44201-SH 20 µg $520
 shRNA Lentiviral Particles (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
c-Jun shRNA (h) Lentiviral Particles sc-29223-V 200 µl $625
c-Jun shRNA (m) Lentiviral Particles sc-29224-V 200 µl $625
c-Jun shRNA (h2) Lentiviral Particles sc-44201-V 200 µl $625
 WB Positive Control Cell Lysates (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
NIH/3T3 + PMA nuclear extract sc-2125 1000 µg $143
Jurkat nuclear extract sc-2132 1000 µg $143
HeLa + UV Cell Lysate sc-2221 500 µg/200 µl $104
NIH/3T3 Whole Cell Lysate sc-2210 500 µg/200 µl $104

c-Jun Background Information
Genes belonging to the Jun and Fos oncogene families encode nuclear proteins that are associated with a number of transcriptional complexes. The c-Jun protein is a major component of the transcription factor AP-1, originally shown to mediate phorbol ester tumor promoter (TPA)-induced expression of responsive genes through the TPA-response element (TRE). The Jun proteins form homo- and heterodimers which bind the TRE, while Fos proteins are active only as heterodimers with any of the Jun proteins. Fos/Jun heterodimers have a much higher affinity for the TRE than Jun homodimers. Ha-Ras augments c-Jun activity and stimulates phosphorylation of its activation domain. An inhibitor of Fos/Jun function, termed IP-1, associates with Fos and Jun and is inactivated upon phosphorylation induced by the cAMP-dependent protein kinase A (PKA).

c-Jun (N) Product Citations
See how others have used c-Jun (N): sc-45 antibody and or c-Jun (N) antibody conjugates.


200 total citations
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c-Jun (N)
Click on image to enlarge
Western blot analysis of c-Jun p39 expression in untreated (A,C) and phorbol ester-induced (B,D) NIH/3T3 nuclear extracts. Antibodies tested include c-Jun (D): sc-44 (A,B) and c-Jun (N): sc-45 (C,D).
c-Jun (N): sc-45. Western blot analysis of c-Jun expression in non-transfected (A) and human c-Jun transfected (B) CHO whole cell lysates.
c-Jun (N): sc-45. Western blot analysis of c-Jun expression in non-transfected: sc-110760 (A) and human c-Jun transfected: sc-110759 (B) 293 whole cell lysates.
c-Jun (N): sc-45. Western blot analysis of c-Jun expression in non-transfected: sc-117752 (A) and mouse c-Jun transfected: sc-125069 (B) 293T whole cell lysates.
c-Jun (N): sc-45. Western blot analysis of c-Jun expression in non-transfected: sc-117752 (A) and mouse c-Jun transfected: sc-125070 (B) 293T whole cell lysates.
c-Jun (N): sc-45. Western blot analysis of c-Jun expression in non-transfected 293T: sc-117752 (A), mouse c-Jun transfected 293T: sc-125071 (B) and NIH/3T3 (C) whole cell lysates.
c-Jun (N): sc-45. Western blot analysis of c-Jun expression in non-transfected CHO: sc-117750 (A), human c-Jun transfected CHO: sc-110019 (B) and NIH/3T3 (C) whole cell lysates.
Western blot analysis of human recombinant c-Jun (A,C) and human recombinant c-Jun phosphorylated by human recombinant JNK1 (B,D) fusion proteins. Antibodies tested include c-Jun (N): sc-45 (A,B) and p-c-Jun (KM-1): sc-822 (C,D).
Anisomycin: sc-3524. Intracellular FCM analysis of Anisomycin (sc-3524) induced c-Jun serine-63 phosphorylation: Pink dotted lines represent rabbit IgG PE stained Anisomycin (sc-3524) treated NIH/3T3 cells. Green line histograms represent untreated NIH/3T3 cells. Solid orange histograms represent Anisomycin (sc-3524) treated NIH/3T3 cells. Antibody tested: c-Jun (N) PE: sc-45 PE.
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