RNase Inhibitor: sc-43504

RNase Inhibitor is a pivotal biochemical agent used predominantly in molecular biology research to inhibit the activity of ribonucleases (RNases), which are enzymes that degrade RNA. This inhibition is crucial for experiments where RNA integrity is essential, such as in cDNA synthesis, RT-PCR, and RNA sequencing. The mechanism of action of RNase Inhibitor involves the formation of a tight, non-covalent complex with RNases, particularly RNase A-type enzymes. This binding effectively blocks the catalytic site of the enzyme, preventing the cleavage of RNA substrates. This inhibitor is typically derived from various sources, including human placenta or recombinant strains of Escherichia coli, and is active across a wide range of pH and temperature conditions but sensitive to DTT and other reducing agents. In the laboratory, RNase Inhibitor is used to stabilize RNA samples by preventing degradation, ensuring that the RNA remains intact for subsequent analysis or manipulation. Its role is critical in maintaining the fidelity of RNA in preparations that would otherwise be susceptible to rapid degradation by ubiquitous RNases, thereby facilitating accurate experimental results and conclusions in RNA-based research methodologies. This tool is indispensable for researchers aiming to explore gene expression, regulate RNA functions, or investigate the molecular underpinnings of RNA-related processes.