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- mouse monoclonal IgG1; 200 µg/ml
- epitope corresponding to amino acids 120-150 of Rho A of human origin
- recommended for detection of Rho A of mouse, rat and human origin by WB, IP, IF and FCM
- agarose conjugate for IP studies, sc-418 AC, 500 µg/0.25 ml agarose
- phycoerythrin (Rho A (26C4) PE: sc-418 PE) conjugate for intracellular FCM, 100 tests
- fluorescein (Rho A (26C4) FITC: sc-418 FITC) and rhodamine (Rho A (26C4) TRITC: sc-418 TRITC) conjugates for immunofluorescence, 200 µg/ml
- Alexa Fluor® 405 (sc-418 AF405), Alexa Fluor® 488 (sc-418 AF488) and Alexa Fluor® 647 (sc-418 AF647) conjugates for FCM or immunofluorescence; 100 µg/2 ml
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Ordering InformationProduct Citations
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| Species |
Gene Name |
Gene ID |
Chromosome Location |
Isoform (mRNA) Accession # |
Protein Accession # |
OMIM™ Number |
| Human |
RHOA |
387 |
3p21.31 |
NM_001664 |
P61586
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165390 |
| Mouse |
Rhoa |
11848 |
9 F2 |
|
Q9QUI0
|
N/A |
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Rho A Background Information
The Ras p21 family of guanine nucleotide proteins has been widely studied in view of its apparent role in signal transduction pathways and high frequency of mutations in human malignancies. It is now clear, however, that the Ras proteins (H-, K- and N-Ras p21) are members of a much larger superfamily of related proteins. Six members of this family, Rap 1A, Rap 1B, Rap 2, R-Ras, Ral A and Ral B, exhibit approximately 50% amino acid homology to Ras. The six mammalian Rho proteins (Rho A, B, C, G, 7 and 8) are approximately 30% homologous to Ras and are expressed in a wide range of cell types. Both Ras p21 and Rho p21, as well as other members of the Ras superfamily, contain a carboxy-terminal CAAX sequence (C, cysteine; A, aliphatic amino acid; X, any amino acid) which in the case of Ras has been shown to be essential for correct localization and function.
| Rho A (26C4) Product Citations |
See how others have used Rho A (26C4): sc-418 antibody and or Rho A (26C4) antibody conjugates.
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Rho A (26C4)
Click on image to enlarge
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Rho A (26C4): sc-418. Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing cytoplasmic localization.
Rho A (26C4): sc-418. Cytoplasmic immunofluorescence staining of methanol-fixed NIH/3T3 fibroblasts.
Rho A (26C4): sc-418. Western blot analysis of Rho A expression in KNRK (A), PC-12 (B), HL-60 (C), HeLa (D), SK-BR-3 (E) and MCF7 (F) whole cell lysates.
Western blot analysis of Rho A expression in A-431 (A), HeLa (B) and KNRK (C) whole cell lysates. Antibodies tested include Rho A (119): sc-179 (A) and Rho A (26C4): sc-418 (B,C). Rho A (26C4) PE: sc-418 PE. Intracellular FCM analysis of methanol permeabilized HeLa cells. Solid black line histogram represents control mouse IgG1.
Rho A (26C4) Alexa Fluor 488: sc-418 AF488. Intracellular FCM analysis of fixed and permeabilized HeLa cells. Black line histogram represents the isotype control, normal mouse IgG 1: sc-3890. Rho A (26C4) Alexa Fluor 647: sc-418 AF647. Intracellular FCM analysis of fixed and permeabilized HeLa cells. Black line histogram represents the isotype control, normal mouse IgG 1: sc-24636. Rho A (26C4): sc-418. Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing cytoplasmic localization using indirect FITC (A) staining and HeLa cells using direct Alexa Fluor 488 (B) staining.
Rho A (26C4): sc-418. Western blot analysis of Rho C expression in non-transfected 293T: sc-117752 (A), human Rho C transfected 293T: sc-116465 (B) and Jurkat (C) whole cell lysates. Rho A (26C4): sc-418. Western blot analysis of Rho A expression in non-transfected: sc-117752 (A) and mouse Rho A transfected: sc-123116 (B) 293T whole cell lysates and mouse brain tissue extract (C). Rho A (26C4): sc-418. Western blot analysis of Rho C expression in non-transfected 293T: sc-117752 (A), mouse Rho C transfected 293T: sc-123119 (B) and Jurkat (C) whole cell lysates. |
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