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- rabbit polyclonal IgG, 200 µg/ml; also available as goat IgG, 200 µg/ml, sc-408-G
- epitope mapping at the C-terminus of A-Raf of human origin
- recommended for detection of A-Raf p68 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including equine, canine, bovine and porcine
- blocking peptide, sc-408 P
- agarose conjugate for IP studies, sc-408 AC, 500 µg/0.25 ml agarose
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Ordering InformationProduct Citations
Recommended Support Products:
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| Species |
Gene Name |
Gene ID |
Chromosome Location |
Isoform (mRNA) Accession # |
Protein Accession # |
OMIM™ Number |
| Human |
ARAF |
369 |
Xp11.23 |
NM_001654 |
P10398
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311010 |
| Mouse |
Araf |
11836 |
X A1.3 |
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P04627
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N/A |
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A-Raf Background Information
Several serine/threonine protein kinases have been implicated as intermediates in signal transduction pathways. These include ERK/MAP kinases, ribosomal S6 kinase (Rsk) and Raf-1. Raf-1 is a cytoplasmic protein with intrinsic serine/ threonine activity. It is broadly expressed in nearly all cell lines tested to date and is the cellular homolog of v-Raf, the product of the transforming gene of the 3611 strain of murine sarcoma virus. The unregulated kinase activity of the v-Raf protein has been associated with transformation and mitogenesis while the activity of Raf-1 is normally suppressed by a regulatory N-terminal domain. A-Raf, a second member of the Raf gene family of serine/threonine protein kinases, exhibits substantial homology to Raf-1 within the kinase domain of the two molecules, but less homology elsewhere. Expression of A-Raf is found at highest levels in urogenital tissues and kidney and at lowest level in brain tissue.
| A-Raf (C-20) Product Citations |
See how others have used A-Raf (C-20): sc-408 antibody and or A-Raf (C-20) antibody conjugates.
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A-Raf (C-20)
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A-Raf (C-20): sc-408. Western blot analysis of A-Raf expression in K-562 whole cell lysate.
A-Raf siRNA (h): sc-29615. Western blot analysis of A-Raf expression in non-transfected control (A) and A-Raf transfected (B) HeLa cells. Blot probed with A-Raf (C-20): sc-408. α-actinin (H-2): sc-17829 used as specificity and loading control. A-Raf (C-20): sc-408. Western blot analysis of A-Raf expression in non-transfected: sc-117752 (A) and human A-Raf transfected: sc-158202 (B) 293T whole cell lysates. |
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