Myc Antibody (9E10): sc-40

c-Myc Antibody (9E10) is a mouse monoclonal IgG1 kappa light chain antibody raised against human c-Myc protein. c-Myc monoclonal antibody (9E10) specifically detects the c-Myc protein across multiple species, including mouse, rat, human, monkey, feline, and canine. Anti-c-Myc monoclonal antibody (9E10) is recommended for use in various applications such as Western blot (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry on paraffin-embedded sections (IHC-P), flow cytometry (FCM), and ELISA. c-Myc (9E10) antibody recognizes the epitope located within amino acids 408-439 of the human c-Myc protein, encompassing the EQKLISEEDL sequence commonly used as an epitope tag in recombinant protein expression. Available in both non-conjugated and multiple conjugated forms, including agarose, HRP, PE, FITC, and various Alexa Fluor® conjugates, c-Myc (9E10) monoclonal antibody is versatile for detecting c-Myc and c-Myc-tagged proteins. The c-Myc family of proteins, which includes c-Myc, N-Myc, and L-Myc, plays crucial roles in cell proliferation, differentiation, and neoplastic transformation. Amplification and overexpression of the c-Myc gene are implicated in various human cancers such as lung, breast, and colon carcinomas, making c-Myc (9E10) antibody an essential reagent in cancer research. The c-Myc protein contains key motifs in its C-terminal region, including a leucine zipper, helix-loop-helix, and basic region, which facilitate sequence-specific DNA binding and protein-protein interactions. c-Myc forms heterodimers with Max, a bHLH-Zip protein, to regulate gene transcription. Max can also interact with other proteins like Mad1 and Mxi1 to repress transcription, highlighting the complex regulatory network involving c-Myc. c-Myc mouse monoclonal antibody (9E10) thus serves as a valuable asset for studying these critical molecular pathways.

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Myc Antibody (9E10) References:

1. Transcriptional activation by the human c-Myc oncoprotein in yeast requires interaction with Max.  |  Amati, B., et al. 1992. Nature. 359: 423-6. PMID: 1406955
2. Myc family oncoproteins function through a common pathway to transform normal cells in culture: cross-interference by Max and trans-acting dominant mutants.  |  Mukherjee, B., et al. 1992. Genes Dev. 6: 1480-92. PMID: 1644290
3. Apoptosis and differentiation of human embryonic stem cells induced by sustained activation of c-Myc.  |  Sumi, T., et al. 2007. Oncogene. 26: 5564-76. PMID: 17369859
4. Myc deletion rescues Apc deficiency in the small intestine.  |  Sansom, OJ., et al. 2007. Nature. 446: 676-9. PMID: 17377531
5. Max: a helix-loop-helix zipper protein that forms a sequence-specific DNA-binding complex with Myc.  |  Blackwood, EM. and Eisenman, RN. 1991. Science. 251: 1211-7. PMID: 2006410
6. Enhanced expression of the N-myc gene in Wilms' tumors.  |  Nisen, PD., et al. 1986. Cancer Res. 46: 6217-22. PMID: 2430692
7. L-myc, a new myc-related gene amplified and expressed in human small cell lung cancer.  |  Nau, MM., et al. Nature. 318: 69-73. PMID: 2997622
8. c-Myc-driven Hepatocarcinogenesis.  |  Moon, H., et al. 2021. Anticancer Res. 41: 4937-4946. PMID: 34593441
9. Target c-Myc to treat pancreatic cancer.  |  Ala, M. 2022. Cancer Biol Ther. 23: 34-50. PMID: 34978469
10. Homogeneously staining chromosomal regions contain amplified copies of an abundantly expressed cellular oncogene (c-myc) in malignant neuroendocrine cells from a human colon carcinoma.  |  Alitalo, K., et al. 1983. Proc Natl Acad Sci U S A. 80: 1707-11. PMID: 6300869
11. Mad-Max transcriptional repression is mediated by ternary complex formation with mammalian homologs of yeast repressor Sin3.  |  Ayer, DE., et al. 1995. Cell. 80: 767-76. PMID: 7889570
12. The many roles of c-Myc in apoptosis.  |  Thompson, EB. 1998. Annu Rev Physiol. 60: 575-600. PMID: 9558477

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Myc Antibody (9E10) has 9,825 citations in a variety of scientific publications.

Primary antibodies like Anti-Myc Antibody (9E10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Myc Antibody (9E10) Specifications

Alternate Product Name: Myc

Product Clone Name: 9E10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 408-439

Epitope Target: c-Myc

Epitope Species: human

Product Reactivity/Detection: c-Myc p67 and c-Myc tagged fusion proteins

Species Reactivity/Detection: mouse, rat, human, monkey, feline, canine

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), FCM, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 9,825 citations in various scientific publications

Available Conjugates/Forms for Myc Antibody (9E10): AC: Agarose, AF405: Alexa Fluor^® 405, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, B: Biotin, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, PerCP: Peridinin Chlorophyll Protein Complex, TRITC: Rhodamine, X: TransCruz Reagent