 |
- mouse monoclonal IgG1; 200 µg/ml
- epitope corresponding to amino acids 408-439 within the carboxy terminal domain of c-Myc of human origin
- recommended for detection of c-Myc p67 of mouse, rat, human and monkey origin by WB, IP, IF, IHC(P), FCM and ELISA; also reactive with additional species, including feline and canine
- widely used in combination with eukaryotic expression vectors encoding proteins with c-Myc (amino acids 408-439) epitope tag (see pCruzTM Expression Vectors )
- agarose conjugate for IP studies, sc-40 AC, 500 µg/0.25 ml agarose
- TransCruz reagent for ChIP application, sc-40 X, 200 µg/0.1 ml
- HRP conjugate, sc-40 HRP, 200 µg/ml
- biotin conjugate, sc-40 B, 200 µg/ml
- fluorescein (sc-40 FITC) and rhodamine (sc-40 TRITC) conjugates for immunofluorescence, 200 µg/ml, phycoerythrin (sc-40 PE), PerCP (sc-40 PerCP) and PerCP-Cy5.5 (sc-40 PCPC5) conjugates for FCM, 100 tests
- Alexa Fluor® 405 (sc-40 AF405), Alexa Fluor® 488 (sc-40 AF488) and Alexa Fluor® 647 (sc-40 AF647) conjugates for FCM or immunofluorescence; 100 µg/2 ml
|
|
| |
Ordering InformationProduct Citations
Recommended Support Products:
(click button of application of choice)
| |
| Species |
Gene Name |
Gene ID |
Chromosome Location |
Isoform (mRNA) Accession # |
Protein Accession # |
OMIM™ Number |
| Human |
MYC |
4609 |
8q24.21 |
NM_002467 |
P01106
|
190080 |
| Mouse |
Myc |
17869 |
15 D1 |
|
P01108
|
N/A |
| |
c-Myc Background Information
c-Myc-, N-Myc- and L-Myc-encoded proteins function in cell proliferation, differentiation and neoplastic disease. Myc proteins are nuclear proteins with relatively short half lives. Amplification of the c-Myc gene has been found in several types of human tumors including lung, breast and colon carcinomas, while the N-Myc gene has been found amplified in neuroblastomas. The L-Myc gene has been reported to be amplified and expressed at high level in human small cell lung carcinomas. The presence of three sequence motifs in the c-Myc COOH terminus, including the leucine zipper, the helix-loop-helix and a basic region provided initial evidence for a sequence-specific binding function. A basic region helix-loop-helix leucine zipper motif (bHLH-Zip) protein, designated Max, specifically associates with c-Myc, N-Myc and L-Myc proteins. The Myc-Max complex binds to DNA in a sequence-specific manner under conditions where neither Max nor Myc exhibit appreciable binding. Max can also form heterodimers with at least two additional bHLH-Zip proteins, Mad and Mxi1, and Mad-Max dimers have been shown to repress transcription through interaction with mSin3.
| c-Myc (9E10) Product Citations |
See how others have used c-Myc (9E10): sc-40 antibody and or c-Myc (9E10) antibody conjugates.
1282 total citations Loading citations.
|
| |
| Alexa Fluor® is a
trademark of Molecular Probes Inc., OR., USA |
|
| |
|
|
|
c-Myc (9E10)
Click on image to enlarge
|
Western blot analysis of c-Myc expression in HeLa (A,D,F), Jurkat (B), K-562 (C) and NIH/3T3 (G) whole cell lysates and Jurkat (E,H) nuclear extracts. Antibodies tested include c-Myc (9E10): sc-40 (A-C), c-Myc (C-33): sc-42 (D,E) and c-Myc (N-262): sc-764 (F-H). c-Myc (9E10): sc-40. Immunoperoxidase staining of formalin-fixed, paraffin-embedded normal human colon showing intense nuclear staining.
c-Myc (9E10): sc-40. Western blot analysis of whole cell lysates prepared from COS cells transfected with a c-Myc fusion protein (A) and non-transfected (B) cells.
c-Myc (9E10) FITC: sc-40 FITC. Intracellular FCM analysis of fixed and permeabilized HeLa cells. Black line histogram represents the isotype control, normal mouse IgG 1: sc-2855. c-Myc (9E10): sc-40. Immunofluorescence staining of methanol-fixed COS cells transfected with c-Myc fusion protein showing cytoplasmic staining.
pCruz Myc: sc-5043. Western blot analysis of c-Myc-Lac Z fusion protein expression in COS cells transfected with pCruz Myc-Lac Z (A), pCruz His-Lac Z (B) and non-transfected COS cells (C). Blot probed with c-Myc (9E10): sc-40. c-Myc siRNA (h): sc-29226. Western blot analysis of c-Myc expression in non-transfected control (A) and c-Myc siRNA transfected (B) HeLa cells. Blot probed with c-Myc (9E10): sc-40. Actin (I-19): sc-1616 used as specificity and loading control. c-Myc (9E10) Alexa Fluor 488: sc-40 AF488. Intracellular FCM analysis of fixed and permeabilized Jurkat cells. Black line histogram represents the isotype control, normal mouse IgG 1: sc-3890. c-Myc (9E10) Alexa Fluor 647: sc-40 AF647. Intracellular FCM analysis of fixed and permeabilized Jurkat cells. Black line histogram represents the isotype control, normal mouse IgG 1: sc-24636. c-Myc (9E10): sc-40. Western blot analysis of c-Myc expression in non-transfected: sc-117752 (A) and human c-Myc transfected: sc-110502 (B) 293T whole cell lysates. c-Myc (9E10): sc-40. Western blot analysis of c-Myc expression in non-transfected: sc-117752 (A) and human c-Myc transfected: sc-116671 (B) 293T whole cell lysates. c-Myc (9E10): sc-40. Western blot analysis of c-Myc expression in non-transfected: sc-117752 (A) and human c-Myc transfected: sc-110502 (B) 293T whole cell lysates. c-Myc (9E10): sc-40. Immunofluorescence staining of methanol-fixed untransfected (A) and c-myc tag transfected HEK 293T cells (B).
Western blot analysis of c-Myc expression in untreated (A) and (E)-4-Hydroxyhexenal ( sc-202593) treated (B) Jurkat whole cell lysates. Antibody tested is c-Myc (9E10): sc-40 (A,B). Note down regulation of c-Myc in lane B. |
|
Download
|
|
