This product has been titrated for optimal performance. Recommended use is 20 uL per test (1x106 cells).
recommended for use with either a single or dual laser Flow Cytometer fitted with appropriate acquisition and analysis software
The flow cytometer must be equipped with a 488 nm laser and must be capable of detecting light scatter (forward and side) and two-color fluorescence with emission detectable in two ranges: 515-545 nm and 562-607 nm.
isotype matched negative control available that includes: rat IgG2a FITC/rat IgG2a PE, sc-3983 CON
