This product has been titrated for optimal performance. Recommended use is 20 uL per test (1x106 cells).
recommended for use with either a single or dual laser Flow Cytometer fitted with appropriate acquisition and analysis software
The flow cytometer must be equipped with 635 nm and 488 nm lasers and must be capable of detecting light scatter (forward and side) and four-color fluorescence with emission detectable in four ranges: 515-545 nm, 562-607 nm, >650 nm and 652-668 nm, and it must be able to threshold and discriminate using the >650 channel.
isotype matched negative control available that includes: Armenian hamster FITC/rat IgG2a PE/rat IgG PE-Cy5/rat IgG2a APC, sc-3964 CON
