PKAα/β/γ cat Antibody (G-6): sc-390548
- PKAα/β/γ cat Antibody (G-6) is a mouse monoclonal IgG1 κ, cited in 13 publications, provided at 200 µg/ml
- raised against amino acids 231-286 mapping near the C-terminus of PKAα catalytic subunit of human origin
- PKA alpha / beta / gamma cat Antibody (G-6) is recommended for detection of PKAα/β cat of mouse, rat and human origin, and PKAγ cat of human origin by WB, IP, IF and ELISA; also reactive with additional species, including and equine, canine, bovine and porcine
- Anti-PKA alpha / beta / gamma cat Antibody (G-6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for PKAα/β/γ cat Antibody (G-6) for WB applications. These reagents are now offered in bundles with PKAα/β/γ cat Antibody (G-6) (see ordering information below).
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SEE ALSO...
PKAα/β/γ cat Antibody (G-6) is a mouse monoclonal IgG1 kappa light chain antibody designed to detect the catalytic subunits alpha, beta, and gamma of protein kinase A (PKA) across multiple species, including mouse, rat, human, equine, canine, bovine, and porcine, through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). PKAα/β/γ cat monoclonal antibody (G-6) is raised against a precise epitope encompassing amino acids 231-286 near the C-terminus of the human PKAα catalytic subunit, ensuring selective and accurate recognition of PKAα, PKAβ, and PKAγ isoforms. Protein kinase A is a crucial cAMP-dependent enzyme that plays a vital role in regulating diverse cellular functions, including cell proliferation, ion transport, metabolism, and gene transcription. Structurally, PKA exists as a tetrameric holoenzyme composed of two regulatory and two catalytic subunits. Upon binding of the second messenger cyclic AMP (cAMP) to the regulatory subunits, the catalytic subunits are released and translocate to specific locations within the cell, such as the cytoplasm and nucleus. This translocation is essential for PKA′s function, as PKA catalytic subunits phosphorylate target proteins in different cellular compartments, thereby modulating various signaling pathways. In the nucleus, active PKA phosphorylates the cAMP response element-binding protein (CREB) on Serine 133, facilitating the recruitment of transcription factors like TFIIB and TBP1 to initiate gene transcription. The precise localization of PKA catalytic subunits is critical for the spatial and temporal regulation of these signaling events, ensuring that cellular responses are appropriately coordinated and executed. Anti-PKAα/β/γ cat antibody (G-6) enables researchers to effectively study the dynamic localization and functional interactions of PKA within cells, providing valuable insights into PKA′s role in both normal physiological processes and various pathological conditions.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PKAα/β/γ cat Antibody (G-6) | sc-390548 | 200 µg/ml | $322.00 | |||
PKAα/β/γ cat Antibody (G-6): m-IgG Fc BP-HRP Bundle | sc-530187 | 200 µg Ab; 10 µg BP | $361.00 | |||
PKAα/β/γ cat Antibody (G-6): m-IgGκ BP-HRP Bundle | sc-523580 | 200 µg Ab, 40 µg BP | $361.00 | |||
PKAα/β/γ cat Antibody (G-6): m-IgG1 BP-HRP Bundle | sc-543914 | 200 µg Ab; 20 µg BP | $361.00 | |||
PKAα/β/γ cat Antibody (G-6) AC | sc-390548 AC | 500 µg/ml, 25% agarose | $424.00 | |||
PKAα/β/γ cat Antibody (G-6) HRP | sc-390548 HRP | 200 µg/ml | $322.00 | |||
PKAα/β/γ cat Antibody (G-6) FITC | sc-390548 FITC | 200 µg/ml | $336.00 | |||
PKAα/β/γ cat Antibody (G-6) PE | sc-390548 PE | 200 µg/ml | $349.00 | |||
PKAα/β/γ cat Antibody (G-6) Alexa Fluor® 488 | sc-390548 AF488 | 200 µg/ml | $364.00 | |||
PKAα/β/γ cat Antibody (G-6) Alexa Fluor® 546 | sc-390548 AF546 | 200 µg/ml | $364.00 | |||
PKAα/β/γ cat Antibody (G-6) Alexa Fluor® 594 | sc-390548 AF594 | 200 µg/ml | $364.00 | |||
PKAα/β/γ cat Antibody (G-6) Alexa Fluor® 647 | sc-390548 AF647 | 200 µg/ml | $364.00 | |||
PKAα/β/γ cat Antibody (G-6) Alexa Fluor® 680 | sc-390548 AF680 | 200 µg/ml | $364.00 | |||
PKAα/β/γ cat Antibody (G-6) Alexa Fluor® 790 | sc-390548 AF790 | 200 µg/ml | $364.00 |