CLASP1 Antibody (D-8): sc-390159
- CLASP1 Antibody (D-8) is a mouse monoclonal IgG1 κ CLASP1 antibody provided at 200 µg/ml
- raised against amino acids 26-95 mapping near the N-terminus of CLASP1 of human origin
- CLASP1 Antibody (D-8) is recommended for detection of CLASP1 of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including and equine, canine and bovine
- Anti-CLASP1 Antibody (D-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for CLASP1 Antibody (D-8) for WB applications. These reagents are now offered in bundles with CLASP1 Antibody (D-8) (see ordering information below).
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CLASP1 Antibody (D-8) is a mouse monoclonal IgG1 kappa light chain antibody that detects CLASP1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-CLASP1 antibody (D-8) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. CLASP1 plays a crucial role in the stabilization of microtubules, particularly during cell division, by binding to the plus ends of microtubules and interacting with CLIP proteins. This interaction is vital for maintaining the integrity of the mitotic spindle, as CLASP1 localizes near the distal end of growing spindle microtubules during mitosis and is a key component of the outer corona region of kinetochores. By promoting pauses in microtubule dynamics and restricting excessive growth, CLASP1 ensures proper chromosome alignment and segregation. Defects in CLASP1 can lead to severe mitotic errors, including spindle collapse and improper kinetochore attachment, which can have significant implications for cell division and overall cellular health.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
CLASP1 Antibody (D-8) References:
- Mast, a conserved microtubule-associated protein required for bipolar mitotic spindle organization. | Lemos, CL., et al. 2000. EMBO J. 19: 3668-82. PMID: 10899121
- Clasps are CLIP-115 and -170 associating proteins involved in the regional regulation of microtubule dynamics in motile fibroblasts. | Akhmanova, A., et al. 2001. Cell. 104: 923-35. PMID: 11290329
- Human CLASP1 is an outer kinetochore component that regulates spindle microtubule dynamics. | Maiato, H., et al. 2003. Cell. 113: 891-904. PMID: 12837247
- How do kinetochores CLASP dynamic microtubules? | Maiato, H., et al. 2003. Cell Cycle. 2: 511-4. PMID: 14504462
- CLASP1 and CLASP2 bind to EB1 and regulate microtubule plus-end dynamics at the cell cortex. | Mimori-Kiyosue, Y., et al. 2005. J Cell Biol. 168: 141-53. PMID: 15631994
- Up-regulation of the ubiquitous alternative splicing factor Tra2beta causes inclusion of a germ cell-specific exon. | Venables, JP., et al. 2005. Hum Mol Genet. 14: 2289-303. PMID: 16000324
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CLASP1 Antibody (D-8) | sc-390159 | 200 µg/ml | $322.00 | |||
CLASP1 Antibody (D-8): m-IgGκ BP-HRP Bundle | sc-523431 | 200 µg Ab, 40 µg BP | $361.00 | |||
CLASP1 Antibody (D-8): m-IgG1 BP-HRP Bundle | sc-533580 | 200 µg Ab; 20 µg BP | $361.00 | |||
CLASP1 Antibody (D-8) AC | sc-390159 AC | 500 µg/ml, 25% agarose | $424.00 | |||
CLASP1 Antibody (D-8) HRP | sc-390159 HRP | 200 µg/ml | $322.00 | |||
CLASP1 Antibody (D-8) FITC | sc-390159 FITC | 200 µg/ml | $336.00 | |||
CLASP1 Antibody (D-8) PE | sc-390159 PE | 200 µg/ml | $349.00 | |||
CLASP1 Antibody (D-8) Alexa Fluor® 488 | sc-390159 AF488 | 200 µg/ml | $364.00 | |||
CLASP1 Antibody (D-8) Alexa Fluor® 546 | sc-390159 AF546 | 200 µg/ml | $364.00 | |||
CLASP1 Antibody (D-8) Alexa Fluor® 594 | sc-390159 AF594 | 200 µg/ml | $364.00 | |||
CLASP1 Antibody (D-8) Alexa Fluor® 647 | sc-390159 AF647 | 200 µg/ml | $364.00 | |||
CLASP1 Antibody (D-8) Alexa Fluor® 680 | sc-390159 AF680 | 200 µg/ml | $364.00 | |||
CLASP1 Antibody (D-8) Alexa Fluor® 790 | sc-390159 AF790 | 200 µg/ml | $364.00 |