PISD Antibody (H-2): sc-390070

PISD Antibody (H-2) is a mouse monoclonal IgM antibody that detects PISD protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-PISD antibody (H-2) is available as the non-conjugated form. PISD protein, also known as phosphatidylserine decarboxylase proenzyme, plays a crucial role in lipid metabolism by catalyzing the conversion of phosphatidylserine to phosphatidylethanolamine, a vital phospholipid component of cellular membranes. This enzymatic activity is particularly important because phosphatidylethanolamine is essential for maintaining membrane integrity and fluidity, influencing various cellular processes such as apoptosis, cell signaling, and membrane fusion. PISD is localized to the inner mitochondrial membrane, where PISD contributes to the regulation of mitochondrial function and energy production. The gene encoding PISD is located on human chromosome 22q12.2, and PISD consists of 408 amino acids, featuring a conserved LGST motif that is cleaved to generate two isoforms, PISD alpha and PISD beta. Notably, PISD can form heterodimers and is highly expressed in liver and testis, highlighting PISD′s significance in both metabolic and reproductive functions.

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PISD Antibody (H-2) References:

1. Disruption of the phosphatidylserine decarboxylase gene in mice causes embryonic lethality and mitochondrial defects.  |  Steenbergen, R., et al. 2005. J Biol Chem. 280: 40032-40. PMID: 16192276
2. High-throughput mass spectrometry screening for inhibitors of phosphatidylserine decarboxylase.  |  Forbes, CD., et al. 2007. J Biomol Screen. 12: 628-34. PMID: 17478478
3. Phosphatidylserine decarboxylases, key enzymes of lipid metabolism.  |  Schuiki, I. and Daum, G. 2009. IUBMB Life. 61: 151-62. PMID: 19165886
4. A cloned gene encoding phosphatidylserine decarboxylase complements the phosphatidylserine biosynthetic defect of a Chinese hamster ovary cell mutant.  |  Kuge, O., et al. 1991. J Biol Chem. 266: 6370-6. PMID: 2007589
5. The homozygous variant c.797G>A/p.(Cys266Tyr) in PISD is associated with a Spondyloepimetaphyseal dysplasia with large epiphyses and disturbed mitochondrial function.  |  Girisha, KM., et al. 2019. Hum Mutat. 40: 299-309. PMID: 30488656
6. PISD is a mitochondrial disease gene causing skeletal dysplasia, cataracts, and white matter changes.  |  Zhao, T., et al. 2019. Life Sci Alliance. 2: PMID: 30858161
7. Knockdown of TFAM in Tumor Cells Retarded Autophagic Flux through Regulating p53 Acetylation and PISD Expression.  |  Jiang, X. and Wang, J. 2020. Cancers (Basel). 12: PMID: 32093281
8. Post-irradiation endolymphatic hydrops vs. post-irradiation sudden deafness.  |  Tien, CT., et al. 2022. Radiother Oncol. 176: 222-227. PMID: 36265683
9. PCBP1 protects bladder cancer cells from mitochondria injury and ferroptosis by inducing LACTB mRNA degradation.  |  Luo, Y., et al. 2023. Mol Carcinog. 62: 907-919. PMID: 37157950
10. Phosphatidylserine decarboxylase downregulation in uric acid‑induced hepatic mitochondrial dysfunction and apoptosis.  |  Liu, N., et al. 2023. MedComm (2020). 4: e336. PMID: 37502610
11. Novel biallelic PISD missense variants cause spondyloepimetaphyseal dysplasia with disproportionate short stature and fragmented mitochondrial morphology.  |  Aagaard Nolting, L., et al. 2024. Clin Genet. 106: 360-366. PMID: 38801004
12. Post-translational processing of the phosphatidylserine decarboxylase gene product in Chinese hamster ovary cells.  |  Kuge, O., et al. 1996. Biochem J. 319 (Pt 1): 33-8. PMID: 8870646

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PISD Antibody (H-2) has 4 citations in a variety of scientific publications.

Primary antibodies like Anti-PISD Antibody (H-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PISD Antibody (H-2) Specifications

Alternate Product Name: PISD

Product Clone Name: H-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgM kappa light chain

Amino Acids: 249-283

Epitope Target: PISD

Epitope Species: human

Product Reactivity/Detection: PISD

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine, bovine, porcine and avian

Additional Non-Mammalian Reactivity: avian

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgM kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 4 citations in various scientific publications

Available Conjugates/Forms for PISD Antibody (H-2): P: Blocking Peptide