EF-2 Antibody (H-8): sc-390014
- EF-2 Antibody (H-8) is a mouse monoclonal IgG2b κ provided at 200 µg/ml
- specific for an epitope mapping between amino acids 811-849 near the C-terminus of EF-2 of human origin
- recommended for detection of EF-2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for EF-2 Antibody (H-8) for WB and IHC(P) applications. These reagents are now offered in bundles with EF-2 Antibody (H-8) (see ordering information below).
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EF-2 Antibody (H-8) is a mouse monoclonal IgG2b antibody that detects EF-2 in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). EF-2, or elongation factor 2, is a crucial GTPase that plays a vital role in the elongation phase of protein biosynthesis on the ribosome, facilitating the translocation of tRNAs and mRNAs during translation. This process is essential for the accurate synthesis of proteins, as EF-2 ensures that the ribosome moves along the mRNA strand, exposing new codons for decoding, which is critical for maintaining the fidelity of protein production. The proper functioning of EF-2 is not only important for cellular growth and function but also has implications in various diseases, including cancer, where protein synthesis is often dysregulated. Anti-EF-2 antibody (H-8) provides a reliable tool for researchers studying the dynamics of protein synthesis and its regulation in different biological contexts.
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EF-2 Antibody (H-8) References:
- Elongation factor-2 kinase regulates autophagy in human glioblastoma cells. | Wu, H., et al. 2006. Cancer Res. 66: 3015-23. PMID: 16540650
- Elongation factor 2 kinase promotes cell survival by inhibiting protein synthesis without inducing autophagy. | Moore, CE., et al. 2016. Cell Signal. 28: 284-93. PMID: 26795954
- Eukaryotic elongation factor 2 kinase promotes angiogenesis in hepatocellular carcinoma via PI3K/Akt and STAT3. | Zhou, Y., et al. 2020. Int J Cancer. 146: 1383-1395. PMID: 31286509
- Targeting eukaryotic elongation factor-2 kinase suppresses the growth and peritoneal metastasis of ovarian cancer. | Erdogan, MA., et al. 2021. Cell Signal. 81: 109938. PMID: 33539938
- Eukaryotic elongation factor 2 kinase regulates foam cell formation via translation of CD36. | Fernando, S., et al. 2022. FASEB J. 36: e22154. PMID: 35032419
- Elongation factor-2 kinase is a critical determinant of the fate and antitumor immunity of CD8+ T cells. | Das, JK., et al. 2022. Sci Adv. 8: eabl9783. PMID: 35108044
- Eukaryotic elongation factor 2 kinase inhibitor, A484954 inhibits perivascular sympathetic nerve stimulation-induced vasoconstriction in isolated renal artery. | Kodama, T., et al. 2022. Eur J Pharmacol. 926: 175042. PMID: 35598844
- Eukaryotic Elongation Factor 2 Kinase Activity Is Required for the Phenotypes of the Rpl24Bst Mouse. | Knight, JRP., et al. 2022. J Invest Dermatol. 142: 3346-3348.e1. PMID: 35850210
- ADP enhances the allosteric activation of eukaryotic elongation factor 2 kinase by calmodulin. | Piserchio, A., et al. 2023. Proc Natl Acad Sci U S A. 120: e2300902120. PMID: 37068230
- Elongation factor-2 kinase: immunological evidence for the existence of tissue-specific isoforms. | Hait, WN., et al. 1996. FEBS Lett. 397: 55-60. PMID: 8941713
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
EF-2 Antibody (H-8) | sc-390014 | 200 µg/ml | $316.00 | |||
EF-2 Antibody (H-8): m-IgGκ BP-HRP Bundle | sc-535580 | 200 µg Ab; 40 µg BP | $354.00 | |||
EF-2 Antibody (H-8): m-IgG2b BP-HRP Bundle | sc-549450 | 200 µg Ab; 10 µg BP | $354.00 | |||
EF-2 (H-8) Neutralizing Peptide | sc-390014 P | 100 µg/0.5 ml | $68.00 |