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donkey anti-goat IgG, F(ab’)2-APC: sc-3860

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  • supplied at 200 µg in 0.5 ml volume
  • F(ab’)2 fragment secondary antibody
  • allophycocyanin conjugated
  • to be used in immunohistochemical staining at a dilution of 1:100 - 1:400
  • F(ab’)2 fragment secondary antibodies should be used when trying to avoid non-specific secondary antibody binding to Fc receptors on cell surfaces
  • Each vial contains 200 μg goat IgG (pre-adsorbed with mouse and human IgG) in 0.5 ml of PBS containing 0.1% gelatin and 0.1% sodium azide
  • See 2 citations for donkey anti-goat IgG, F(ab’)2-APC
 
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donkey anti-goat IgG, F(ab’)2-APC sc-3860 200 µg/0.5 ml $100

Santa Cruz Biotechnology’s secondary antibodies are available conjugated to either an enzyme, biotin or fluorophore for use in a variety of antibody-based applications including Western Blot, immunostaining, flow cytometry and ELISA. Secondary antibodies are commonly affinity purified against immobilized whole IgG or against antibody fragments such as the Fc or F(ab')2 regions. Santa Cruz Biotechnology offers an extensive selection of F(ab')2 specific secondary antibodies for immunohistochemistry and flow cytometry that are non-conjugated or labeled with either AP (alkaline phosphatase), fluorescein, biotin, FITC (fluorescein isothiocyanate), Texas Red®, TRITC (tetramethyl rhodamine iso-thiocyanate), PE (phycoerythrin), PE-Cy5 (phycoerythrin with cyanin-5), PE-Cy7 (phycoerythrin with cyanin-7), APC (allophycocyanin), APC-Cy7 and (allophycocyanin with cyanin-7). F(ab')2 secondary antibodies are specific for commonly used primary antibody species, including goat, rabbit, mouse and rat, and are recommended for reducing non-specific secondary antibody binding to Fc receptors on the cell surface.

donkey anti-goat IgG, F(ab’)2-APC Product Citations
See how others have used donkey anti-goat IgG, F(ab’)2-APC.


2 total citations
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donkey anti-goat IgG, F(ab’)2-APC Data
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sc-3860
Glucosidase IIβ (N-19): sc-6452. Indirect, intracellular FCM analysis of fixed and permeabilized K-562 cells stained with Glucosidase IIβ (N-19), followed by APC-conjugated donkey anti-goat IgG F(ab')2: sc-3860. Black line histogram represents the isotype control, normal goat IgG: sc-2028.
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