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goat anti-rat IgG, F(ab’)2-APC: sc-3832

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  • supplied at 200 µg in 0.5 ml volume
  • F(ab’)2 fragment secondary antibody
  • allophycocyanin conjugated
  • to be used in immunohistochemical staining at a dilution of 1:100 - 1:400
  • F(ab’)2 fragment secondary antibodies should be used when trying to avoid non-specific secondary antibody binding to Fc receptors on cell surfaces
  • pre-adsorbed with mouse and human IgG
  • See 1 citations for goat anti-rat IgG, F(ab’)2-APC
 
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goat anti-rat IgG, F(ab’)2-APC sc-3832 200 µg/0.5 ml $100

Santa Cruz Biotechnology’s secondary antibodies are available conjugated to either an enzyme, biotin or fluorophore for use in a variety of antibody-based applications including Western Blot, immunostaining, flow cytometry and ELISA. Secondary antibodies are commonly affinity purified against immobilized whole IgG or against antibody fragments such as the Fc or F(ab')2 regions. Santa Cruz Biotechnology offers an extensive selection of F(ab')2 specific secondary antibodies for immunohistochemistry and flow cytometry that are non-conjugated or labeled with either AP (alkaline phosphatase), fluorescein, biotin, FITC (fluorescein isothiocyanate), Texas Red®, TRITC (tetramethyl rhodamine iso-thiocyanate), PE (phycoerythrin), PE-Cy5 (phycoerythrin with cyanin-5), PE-Cy7 (phycoerythrin with cyanin-7), APC (allophycocyanin), APC-Cy7 and (allophycocyanin with cyanin-7). F(ab')2 secondary antibodies are specific for commonly used primary antibody species, including goat, rabbit, mouse and rat, and are recommended for reducing non-specific secondary antibody binding to Fc receptors on the cell surface.

goat anti-rat IgG, F(ab’)2-APC Product Citations
See how others have used goat anti-rat IgG, F(ab’)2-APC.


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goat anti-rat IgG, F(ab’)2-APC Data
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sc-3832
CD2 (AT37): sc-13531. Indirect FCM analysis of TK-1 cells stained with CD2 (AT37), followed by APC-conjugated goat anti-rat IgG F(ab')2: sc-3832. Black line histogram represents the isotype control, normal rat IgG2a: sc-3883.
sc-3832
HCAM (IM7): sc-18849. Indirect FCM analysis of TK-1 cells stained with HCAM (IM7), followed by APC-conjugated goat anti-rat IgG F(ab')2: sc-3832. Black line histogram represents the isotype control, normal rat IgG2b: sc-3884.
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