p-p21 Antibody (D-4) | Santa Cruz Biotech

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p-p21 Antibody (D-4): sc-377515	\| Datasheet

(Based on data analysis)

p-p21 Antibody (D-4) is a mouse monoclonal IgG₃ provided at 200 µg/ml
epitope corresponding to a short amino acid sequence containing phosphorylated Ser 146 of p21 of human origin
recommended for detection of Ser 146 phosphorylated p21 of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including canine, bovine and porcine

See additional p21 Antibodies.

Also see p21 Activators for functional analysis of cellular responses to p21.

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WB IP IF

Species	Gene Name	Gene ID	Chromosome Location	Isoform (mRNA) Accession #	Protein Accession #	OMIM™ Number

Human	CDKN1A	1026	6p21.2	NM_000389, NM_001220777, NM_001220778, NM_078467	P38936	116899
Mouse	Cdkn1a	12575	17 A3.3	NM_001111099, NM_007669	P39689	N/A

The p53-inducible gene product, p21(WAF1/CIP1), plays a critical role in regulating the rate of tumor incidence. p21 is a kinase inhibitor that was originally identified as a cyclin-dependent kinase and PCNA-binding protein able to inhibit CDK catalytic activity and as a gene whose expression was induced by the tumor suppressor protein p53 (2–4,5). p21 contributes to the regulation of cell division, mediation of negative growth signals, differentiation and senescence, modulation of the apoptotic response and activaion of certain cyclin-CDKs in response to mitogenic signals (4,6,7). Serine 146 is a phosphorylation site within the carboxy terminal regulatory domain of p21 and when modified in vivo influences p21-PCNA interactions (1,5).

p-p21 Antibody (D-4) Data

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Western blot analysis of p21 phosphorylation in untreated (A,D), human recombinant PKC α treated (B,E) and human recombinant PKC α and lambda protein phosphatase (sc-200312) treated (C,F) human recombinant p21 fusion proteins. Antibodies tested include p-p21 (D-4): sc-377515 (A,B,C) and p21 (C-19): sc-397 (D,E,F).

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p-p21 Antibody (D-4): sc-377515