SPOP Antibody (B-8): sc-377206

SPOP Antibody (B-8) is a mouse monoclonal IgG2b kappa light chain antibody that detects SPOP protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-SPOP antibody (B-8) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. SPOP protein, also known as speckle-type POZ protein, TEF2, HIB homolog 1, or Roadkill homolog 1, plays a crucial role in cellular processes by mediating ubiquitinylation and protein degradation through interaction with the CUL-3 ubiquitin ligase complex. Structurally, SPOP contains an N-terminal MATH (Meprin and TRAF Homology) domain and a C-terminal BTB/POZ domain, which are essential for function; the MATH domain is responsible for substrate recognition, while the BTB/POZ domain facilitates interaction with CUL-3. This dual-domain architecture allows SPOP to effectively recruit various substrates, including PDX-1, Bmi-1, MacroH2A, PIPK II β, and Daxx, for ubiquitin-mediated degradation, thereby regulating critical cellular pathways. SPOP undergoes ubiquitination and subsequent proteasomal degradation, highlighting a dynamic role in maintaining protein homeostasis within the cell. Nuclear expression of SPOP across various tissues underscores importance in cellular regulation and potential implications in disease processes, making SPOP monoclonal antibody (B-8) a valuable tool for researchers studying these mechanisms.

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SPOP Antibody (B-8) References:

1. A diverse family of proteins containing tumor necrosis factor receptor-associated factor domains.  |  Zapata, JM., et al. 2001. J Biol Chem. 276: 24242-52. PMID: 11279055
2. MacroH2A1.2 binds the nuclear protein Spop.  |  Takahashi, I., et al. 2002. Biochim Biophys Acta. 1591: 63-8. PMID: 12183056
3. Identification of PCIF1, a POZ domain protein that inhibits PDX-1 (MODY4) transcriptional activity.  |  Liu, A., et al. 2004. Mol Cell Biol. 24: 4372-83. PMID: 15121856
4. Daxx-mediated transcriptional repression of MMP1 gene is reversed by SPOP.  |  La, M., et al. 2004. Biochem Biophys Res Commun. 320: 760-5. PMID: 15240113
5. Stable X chromosome inactivation involves the PRC1 Polycomb complex and requires histone MACROH2A1 and the CULLIN3/SPOP ubiquitin E3 ligase.  |  Hernández-Muñoz, I., et al. 2005. Proc Natl Acad Sci U S A. 102: 7635-40. PMID: 15897469
6. BTB domain-containing speckle-type POZ protein (SPOP) serves as an adaptor of Daxx for ubiquitination by Cul3-based ubiquitin ligase.  |  Kwon, JE., et al. 2006. J Biol Chem. 281: 12664-72. PMID: 16524876
7. A hedgehog-induced BTB protein modulates hedgehog signaling by degrading Ci/Gli transcription factor.  |  Zhang, Q., et al. 2006. Dev Cell. 10: 719-29. PMID: 16740475
8. Coordinated activation of the nuclear ubiquitin ligase Cul3-SPOP by the generation of phosphatidylinositol 5-phosphate.  |  Bunce, MW., et al. 2008. J Biol Chem. 283: 8678-86. PMID: 18218622
9. SPOP-mediated ubiquitination and degradation of PDK1 suppresses AKT kinase activity and oncogenic functions.  |  Jiang, Q., et al. 2021. Mol Cancer. 20: 100. PMID: 34353330
10. SPOP mutations promote p62/SQSTM1-dependent autophagy and Nrf2 activation in prostate cancer.  |  Shi, Q., et al. 2022. Cell Death Differ. 29: 1228-1239. PMID: 34987184
11. Deregulation of SPOP in Cancer.  |  Zhang, H., et al. 2023. Cancer Res. 83: 489-499. PMID: 36512624
12. Identification of a novel nuclear speckle-type protein, SPOP.  |  Nagai, Y., et al. 1997. FEBS Lett. 418: 23-6. PMID: 9414087

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-SPOP Antibody (B-8) has 6 citations in a variety of scientific publications.

Primary antibodies like Anti-SPOP Antibody (B-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

SPOP Antibody (B-8) Specifications

Alternate Product Name: Speckle-Type POZ Protein

Product Clone Name: B-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 351-374

Epitope Target: SPOP

Epitope Species: human

Product Reactivity/Detection: SPOP

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine, bovine, porcine and avian

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 6 citations in various scientific publications

Available Conjugates/Forms for SPOP Antibody (B-8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin