Insulin B Antibody (C-12): sc-377071
- Insulin B Antibody (C-12) is a mouse monoclonal IgG1 κ Insulin B antibody, cited in 23 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 33-69 near the N-terminus of Insulin B of human origin
- Insulin B Antibody (C-12) is recommended for detection of Insulin B chain of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and equine, canine, bovine and porcine
- Anti-Insulin B Antibody (C-12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to biotin for WB, IHC(P) and ELISA
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Insulin B Antibody (C-12) for WB and IHC(P) applications. These reagents are now offered in bundles with Insulin B Antibody (C-12) (see ordering information below).
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Insulin B Antibody (C-12) is a mouse monoclonal IgG1 kappa light chain antibody designed to target an epitope spanning amino acids 33-69 near the N-terminus of the human Insulin B chain. Insulin B monoclonal antibody (C-12) exhibits broad species reactivity, effectively recognizing insulin from mouse, rat, and human sources, as well as additional species including equine, canine, bovine, and porcine, making it versatile for various experimental applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-Insulin B antibody (C-12) is available in both non-conjugated form and multiple conjugated forms including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and various Alexa Fluor® dyes, providing flexibility for diverse research needs. Insulin itself is a critical secreted peptide hormone that regulates metabolic processes by increasing glucose uptake and glycogen synthesis, thereby reducing blood glucose levels. Insulin is synthesized initially as preproinsulin, which is subsequently processed to proinsulin and finally to the mature insulin hormone consisting of 51 amino acids divided into an A chain and a B chain connected by two disulfide bridges. Insulin enhances cellular permeability to monosaccharides, amino acids, and fatty acids, playing a vital role in maintaining energy homeostasis. Insulin is secreted by the pancreatic beta cells at basal levels and secretion significantly increases in response to elevated blood glucose levels. Insulin hormone exerts effects by binding to specific insulin receptors on target cell membranes, triggering signaling pathways that facilitate glucose uptake and utilization. Proper insulin function is essential for preventing metabolic disorders, and defects in insulin production or signaling are major causes of hyperproinsulinemia and type-II diabetes mellitus. By providing precise detection of the Insulin B protein, Insulin B (C-12) antibody is an indispensable tool for researchers studying insulin′s role in metabolism, regulatory mechanisms, and involvement in various pathological conditions.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Insulin B Antibody (C-12) References:
- Differential signalling potential of insulin- and IGF-1-receptor cytoplasmic domains. | Lammers, R., et al. 1989. EMBO J. 8: 1369-75. PMID: 2548842
- The molecular mechanism of insulin action. | Kahn, CR. 1985. Annu Rev Med. 36: 429-51. PMID: 2986528
- Solution structure of the superactive monomeric des-[Phe(B25)] human insulin mutant: elucidation of the structural basis for the monomerization of des-[Phe(B25)] insulin and the dimerization of native insulin. | Jørgensen, AM., et al. 1996. J Mol Biol. 257: 684-99. PMID: 8648633
- Cytosolic calcium oscillations and insulin release in pancreatic islets of Langerhans. | Soria, B. and Martin, F. 1998. Diabetes Metab. 24: 37-40. PMID: 9534007
- Proinsulin: recent observations and controversies. | Mackin, RB. 1998. Cell Mol Life Sci. 54: 696-702. PMID: 9711235
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Insulin B Antibody (C-12) | sc-377071 | 200 µg/ml | $316.00 | |||
Insulin B Antibody (C-12): m-IgG Fc BP-HRP Bundle | sc-529966 | 200 µg Ab; 10 µg BP | $354.00 | |||
Insulin B Antibody (C-12): m-IgGκ BP-HRP Bundle | sc-523173 | 200 µg Ab, 40 µg BP | $354.00 | |||
Insulin B Antibody (C-12): m-IgG1 BP-HRP Bundle | sc-543793 | 200 µg Ab; 20 µg BP | $354.00 | |||
Insulin B Antibody (C-12) AC | sc-377071 AC | 500 µg/ml, 25% agarose | $416.00 | |||
Insulin B Antibody (C-12) HRP | sc-377071 HRP | 200 µg/ml | $316.00 | |||
Insulin B Antibody (C-12) FITC | sc-377071 FITC | 200 µg/ml | $330.00 | |||
Insulin B Antibody (C-12) PE | sc-377071 PE | 200 µg/ml | $343.00 | |||
Insulin B Antibody (C-12) Alexa Fluor® 488 | sc-377071 AF488 | 200 µg/ml | $357.00 | |||
Insulin B Antibody (C-12) Alexa Fluor® 546 | sc-377071 AF546 | 200 µg/ml | $357.00 | |||
Insulin B Antibody (C-12) Alexa Fluor® 594 | sc-377071 AF594 | 200 µg/ml | $357.00 | |||
Insulin B Antibody (C-12) Alexa Fluor® 647 | sc-377071 AF647 | 200 µg/ml | $357.00 | |||
Insulin B Antibody (C-12) Alexa Fluor® 680 | sc-377071 AF680 | 200 µg/ml | $357.00 | |||
Insulin B Antibody (C-12) Alexa Fluor® 790 | sc-377071 AF790 | 200 µg/ml | $357.00 | |||
Insulin B Antibody (C-12) B | sc-377071 B | 200 µg/ml | $320.00 | |||
Insulin B (C-12) Neutralizing Peptide | sc-377071 P | 100 µg/0.5 ml | $68.00 |