MKP-3 Antibody (F-12): sc-377070

MKP-3 Antibody (F-12) is a mouse monoclonal IgG1 kappa light chain antibody that detects MKP-3 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). MKP-3 (F-12) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. MKP-3, a member of the dual specificity phosphatase family, plays a crucial role in regulating mitogen-activated protein (MAP) kinase signaling pathways, which are essential for cellular responses to growth factors and stress signals. By specifically dephosphorylating critical phosphothreonine and phosphotyrosine residues within MAP kinases, MKP-3 serves to inactivate members of the extracellular signal-regulated kinase (ERK) family, thereby modulating various physiological processes such as cell proliferation, differentiation, and apoptosis. MKP-3 expression is tightly regulated and can be induced by various stimuli, highlighting MKP-3′s importance in maintaining cellular homeostasis and preventing aberrant signaling that could lead to pathological conditions. Human MKP-3 is located on chromosome 12q22-q23 and encodes a 381 amino acid protein that exhibits the highest expression levels in the heart and pancreas, underscoring MKP-3′s potential significance in these tissues.

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MKP-3 Antibody (F-12) References:

1. Dual specificity phosphatases: a gene family for control of MAP kinase function.  |  Camps, M., et al. 2000. FASEB J. 14: 6-16. PMID: 10627275
2. Nitric oxide down-regulates MKP-3 mRNA levels: involvement in endothelial cell protection from apoptosis.  |  Rössig, L., et al. 2000. J Biol Chem. 275: 25502-7. PMID: 10846176
3. MAP kinase phosphatase 3 (MKP3) interacts with and is phosphorylated by protein kinase CK2alpha.  |  Castelli, M., et al. 2004. J Biol Chem. 279: 44731-9. PMID: 15284227
4. Negative-feedback regulation of FGF signalling by DUSP6/MKP-3 is driven by ERK1/2 and mediated by Ets factor binding to a conserved site within the DUSP6/MKP-3 gene promoter.  |  Ekerot, M., et al. 2008. Biochem J. 412: 287-98. PMID: 18321244
5. Spinal mitogen-activated protein kinase phosphatase-3 (MKP-3) is necessary for the normal resolution of mechanical allodynia in a mouse model of acute postoperative pain.  |  Saha, M., et al. 2013. J Neurosci. 33: 17182-7. PMID: 24155322
6. p53 protein-mediated up-regulation of MAP kinase phosphatase 3 (MKP-3) contributes to the establishment of the cellular senescent phenotype through dephosphorylation of extracellular signal-regulated kinase 1/2 (ERK1/2).  |  Zhang, H., et al. 2015. J Biol Chem. 290: 1129-40. PMID: 25414256
7. ROS elevate HIF-1α phosphorylation for insect lifespan through the CK2-MKP3-p38 pathway.  |  Su, X., et al. 2023. Biochim Biophys Acta Mol Cell Res. 1870: 119389. PMID: 36372111
8. PRSS50-mediated inhibition of MKP3/ERK signaling is crucial for meiotic progression and sperm quality.  |  Niu, CX., et al. 2024. Zool Res. 45: 1037-1047. PMID: 39147718
9. An emerging family of dual specificity MAP kinase phosphatases.  |  Keyse, SM. 1995. Biochim Biophys Acta. 1265: 152-60. PMID: 7696343
10. MKP-3, a novel cytosolic protein-tyrosine phosphatase that exemplifies a new class of mitogen-activated protein kinase phosphatase.  |  Muda, M., et al. 1996. J Biol Chem. 271: 4319-26. PMID: 8626780
11. Molecular cloning and functional characterization of a novel mitogen-activated protein kinase phosphatase, MKP-4.  |  Muda, M., et al. 1997. J Biol Chem. 272: 5141-51. PMID: 9030581
12. Functional studies of dual-specificity phosphatases.  |  Sun, H. 1998. Methods Mol Biol. 84: 307-18. PMID: 9666459

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-MKP-3 Antibody (F-12) has 25 citations in a variety of scientific publications.

Primary antibodies like Anti-MKP-3 Antibody (F-12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

MKP-3 Antibody (F-12) Specifications

Alternate Product Name: MKP-3

Product Clone Name: F-12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 362-381

Epitope Target: MKP-3

Epitope Species: human

Product Reactivity/Detection: MKP-3

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine, bovine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 25 citations in various scientific publications

Available Conjugates/Forms for MKP-3 Antibody (F-12): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin