Int-6 Antibody (D-8) | Santa Cruz Biotech

Welcome to Santa Cruz Biotechnology!

Research Products
Primary Antibodies: Mammalian Phospho-Specific Antibodies Antibodies for IHC (Paraffin) Staining Flow Cytometry Veterinary Research Antibodies Primary Antibodies: Non-Mammalian siRNA and shRNA (plasmid and lentivirus) Products Protein A, G PLUS and L-Agarose Tissue Extracts and Cell Lysates Biologically Active and Purified Proteins Transfected Cell Lysates Secondary Antibodies, Control Antibodies and Normal Sera Specialty Reagents and Kits
Chemicals
Biochemicals Prepared Buffers Buffer Components and Standard Lab Chemicals Stains Dyes and Fluorescent Probes Solvents HRP and AP Substrates
Lab Supplies
Animal Health
Animal Health Promo Animal Health Book Promo Animal Health News Animal Health Pharmacy Ultra Cruz Supplements
What's New
Promotionals LIF Protein Veterinary Research Antibodies Monoclonal Antibodies New IHC (Paraffin) Staining
Information
Contact Us Order Information Cruz Credits™ Investigator Awards Job Opportunities Distributors
Support
Protocols Product Citations Datasheets / MSDS Technical Service Catalog Request Chromosome DBase

Int-6 Antibody (D-8): sc-376899	\| Datasheet

5 4 (Based on data analysis) 1

Int-6 Antibody (D-8) is a mouse monoclonal IgG_2a provided at 200 µg/ml
specific for an epitope mapping between amino acids 415-444 at the C-terminus of Int-6 of mouse origin
recommended for detection of Int-6 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including equine, canine and porcine

See additional Int-6 Antibodies.

Ordering InformationGene Info

Recommended Support Products:

(click button of application of choice)

WB IP IF IHC(P)

Species	Gene Name	Gene ID	Chromosome Location	Isoform (mRNA) Accession #	Protein Accession #	OMIM™ Number

Human	EIF3E	3646	8q23.1	NM_001568	P60228	602210
Mouse	Eif3e	16341	15 B3.2	NM_008388	P60229	N/A

Int-6, also designated eIF3e, eIF3-p46, eIF3-p48 and eukaryotic translation initiation factor 3, subunit 6, regulates translation and protein degradation through binding with three complexes: the eukaryotic translation initiation factor 3 (eIF3), the proteasome regulatory lid and the constitutive photomorphogenesis 9 signalosome. eIF3 is a complex that mediates assembly of 40S ribosomal subunits on mRNA bearing either a 5'-cap or an internal ribosome entry site (IRES). The Int-6 gene is a site of mouse mammary tumour virus (MMTV) integration in murine tumors. Reducing Int-6 expression by RNA interference in HeLa cells alters mitosis progression through defects in spindle formation, chromosome segregation and cytokinesis. These abberations appear to correlate with an inhibition of cyclin B-Cdk1 kinase activity, due to a protracted inhibitory phosphorylated state of Cdk1.

Int-6 Antibody (D-8) Data

Click on image to enlarge

Int-6 (D-8): sc-376899. Western blot analysis of Int-6 expression in NIH/3T3 (A) and KNRK (B) whole cell lysates.

Int-6 (D-8): sc-376899. Immunoperoxidase staining of formalin fixed, paraffin-embedded human esophagus tissue showing cytoplasmic staining of squamous epithelial cells.

Download

Datasheets

Get Adobe Acrobat Reader

Int-6 Antibody (D-8): sc-376899