HURP Antibody (D-12): sc-376760
- HURP Antibody (D-12) is a mouse monoclonal IgG3 κ provided at 200 µg/ml
- specific for an epitope mapping between amino acids 417-451 within an internal region of HURP of human origin
- recommended for detection of HURP of human origin by WB, IP, IF, IHC(P) and ELISA
- m-IgG3 BP-HRP is the preferred secondary detection reagent for HURP Antibody (D-12) for WB and IHC(P) applications. This reagent is now offered in a bundle with HURP Antibody (D-12) (see ordering information below).
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HURP Antibody (D-12) is a mouse monoclonal IgG3 antibody that detects HURP in human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). HURP, also known as Hepatoma up-regulated protein or DLGAP5, is an 846 amino acid protein that plays a crucial role in cell cycle regulation, particularly during the G2/M phase. HURP (D-12) antibody recognizes a protein essential for proper chromosome segregation through localization to spindle poles in mitotic cells, maintaining genomic stability. HURP′s interaction with Cdc2 p34 mediates adherens junction assembly and differentiation in epithelial cells, processes critical for tissue integrity and function. HURP undergoes post-translational modifications, including phosphorylation by ATM or ATR in response to DNA damage, which influences stability and function. Ubiquitin-induced proteasomal degradation regulates HURP levels, highlighting a dynamic role in cellular processes. Two isoforms of HURP arise from alternative splicing, contributing to functional diversity in different cellular contexts.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
HURP Antibody (D-12) References:
- Prognostic and predictive value of HURP in non‑small cell lung cancer. | Wang, Q., et al. 2018. Oncol Rep. 39: 1682-1692. PMID: 29484418
- The PRMT5/HURP axis retards Golgi repositioning by stabilizing acetyl-tubulin and Golgi apparatus during cell migration. | Chiu, SC., et al. 2022. J Cell Physiol. 237: 1033-1043. PMID: 34541678
- Evidence for a HURP/EB free mixed-nucleotide zone in kinetochore-microtubules. | Castrogiovanni, C., et al. 2022. Nat Commun. 13: 4704. PMID: 35948594
- The JMJD6/HURP axis promotes cell migration via NF-κB-dependent centrosome repositioning and Cdc42-mediated Golgi repositioning. | Huang, YJ., et al. 2022. J Cell Physiol. 237: 4517-4530. PMID: 36250981
- The crescent-like Golgi ribbon is shaped by the Ajuba/PRMT5/Aurora-A complex-modified HURP. | Chiu, SC., et al. 2023. Cell Commun Signal. 21: 156. PMID: 37370099
- HURP localization in metaphase is the result of a multi-step process requiring its phosphorylation at Ser627 residue. | Didaskalou, S., et al. 2023. Front Cell Dev Biol. 11: 981425. PMID: 37484914
- Molecular interplay between HURP and Kif18A in mitotic spindle regulation. | Perez-Bertoldi, JM., et al. 2024. bioRxiv.. PMID: 38645125
- HURP binding to the vinca domain of β-tubulin accounts for cancer drug resistance. | Saju, A., et al. 2024. Nat Commun. 15: 8844. PMID: 39397030
- HURP regulates Kif18A recruitment and activity to synergistically control microtubule dynamics. | Perez-Bertoldi, JM., et al. 2024. Nat Commun. 15: 9687. PMID: 39516196
- HURP facilitates spindle assembly by stabilizing microtubules and working synergistically with TPX2. | Valdez, VA., et al. 2024. Nat Commun. 15: 9689. PMID: 39516491
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HURP Antibody (D-12) | sc-376760 | 200 µg/ml | $316.00 | |||
HURP Antibody (D-12): m-IgG3 BP-HRP Bundle | sc-550462 | 200 µg Ab; 40 µg BP | $354.00 | |||
HURP (D-12) Neutralizing Peptide | sc-376760 P | 100 µg/0.5 ml | $68.00 |