HPRT Antibody (A-10): sc-376559

HPRT Antibody (A-10) is a mouse monoclonal IgG1 antibody that detects hypoxanthine phosphoribosyltransferase 1 (HPRT) in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). HPRT is a crucial enzyme in purine metabolism, functioning as a purine salvage enzyme that catalyzes the conversion of hypoxanthine and guanine into their respective mononucleotides, inosine monophosphate and guanosine monophosphate. This enzymatic activity is vital for recycling purines, which are essential building blocks for nucleic acids and energy metabolism. HPRT exists as a homotetramer and requires two magnesium ions as cofactors for its activity, highlighting the importance of metal ion interactions in its function. Deficiencies in HPRT activity can lead to serious metabolic disorders, including gout and Lesch-Nyhan syndrome, underscoring the enzyme′s critical role in maintaining purine homeostasis. Anti-HPRT antibody (A-10) is an invaluable tool for researchers studying these metabolic pathways and their associated diseases, providing insights into the molecular mechanisms underlying purine metabolism and its implications in human health.

The preferred HPRT antibody is HPRT Antibody (F-1): sc-376938.